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Purified anti-TPP1 Antibody

Pricing & Availability
Clone
2E12 (See other available formats)
Regulatory Status
RUO
Other Names
Tripeptidyl Peptidase 1, Tripeptidyl Aminopeptidase, TPP-1, LPIC, Ceroid-Lipofuscinosis, Neuronal 2, Spinocerebellar Ataxia, Autosomal Recessive 7, Tripeptidyl-Peptidase 1, Tripeptidyl-Peptidase I, TPP I , SCAR7, GIG1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
2E12_PURE_TPP1_Antibody_1_032818
Western blot of purified anti-TPP1 antibody (clone 2E12). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate; Lane 3: 20 µg of human frontal cortex lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of mouse brain lysate. The blot was incubated with 5 µg/mL of the primary antibody for 2 hours at room temperature, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 2E12_PURE_TPP1_Antibody_1_032818
    Western blot of purified anti-TPP1 antibody (clone 2E12). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate; Lane 3: 20 µg of human frontal cortex lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of mouse brain lysate. The blot was incubated with 5 µg/mL of the primary antibody for 2 hours at room temperature, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • 2E12_PURE_TPP1_Antibody_2_032818
    IHC staining of purified anti-TPP1 antibody (clone 2E12) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 1 µg/mL of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The image was captured with a 20X objective. Scale bar: 50 µm.
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852201 25 µg $112
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852202 100 µg $276
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Description

TPP1 is a member of the sedolisin family of serine proteases. The proenzyme is activated in the lysosome by auto-proteolysis where it cleaves tripeptides from the N-termini of substrates. Mutations of TPP1 are associated with late-infantile neuronal ceroid lipofuscinosis and Spinocerebellar Ataxia, Autosomal Recessive 7.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant mature human TPP1 expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize rodent (mouse or rat) TPP1.

Application References

(PubMed link indicates BioLegend citation)
  1. Wujek P, et al. 2004. J. Biol. Chem. 279:12827-39.
RRID
AB_2728589 (BioLegend Cat. No. 852201)
AB_2728589 (BioLegend Cat. No. 852202)

Antigen Details

Structure
Human TPP1 is a 563 amino acid protein with a molecular mass of 61 kD. An isoform of 34.5 kD and 320 amino acids (244-563) is generated by alternative splicing.
Distribution

Tissue sources: Ubiquitously expressed. Highest levels in heart, placenta, bone marrow, lung, pineal, and lymphocytes.
Cellular distribution: Lysosome and extracellular.

Function
TPP1 cleaves tripeptides from the N-termini of substrates in lysosomes and has endopeptidase activity.
Interaction
CLN5.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Getty AL, Pearce DA. 2011. Cell Mol. Life Sci. 68:453-74.
  2. Cárcel-Trullols J, et al. 2015. Biochim. Biophys. Acta. 2242-55.
  3. Kyttälä A, et al. 2006. Biochim Biophys Acta. 920-33.
Gene ID
1200 View all products for this Gene ID
UniProt
View information about TPP1 on UniProt.org
Go To Top Version: 1    Revision Date: 03/29/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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