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Purified anti-TrpC5 Antibody

Pricing & Availability
Clone
N67/15 (See other available formats)
Regulatory Status
RUO
Other Names
TRPC5; TRP5; PPP1R159
Isotype
Mouse IgG2b, κ
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Product Citations
publications
N67-15_PURE_TrpC5_Antibody_101921.png
IHC staining of anti-TrpC5 antibody (clone N67/15) on formalin-fixed paraffin-embedded rat eye tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N67-15_PURE_TrpC5_Antibody_101921.png
    IHC staining of anti-TrpC5 antibody (clone N67/15) on formalin-fixed paraffin-embedded rat eye tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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601152 100 µg $165
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Description

TrpC5 is a non-voltage dependent calcium-permeable ion channel in the superfamily of transient receptor potential channels. TrpC5 protein is abundant in the nervous and cardiovascular systems and maintains intracellular Ca2+ levels by forming homotetrameric or heterotetrameric channels with TrpC4 and/or TrpC1. Upon receiving physiological and mechanical stimuli, TrpC5 induces Ca2+ influx and cell membrane depolarization.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial synthetic peptide of human TrpC5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes endogenous TrpC5 proteins on rat eye tissues.

RRID
AB_2904440 (BioLegend Cat. No. 601152)

Antigen Details

Structure
TrpC5 is a 973 amino acid protein with a molecular mass of 111 kD.
Distribution

Membrane/ Retina ganglion cells, endothelial cells, smooth muscle cells

Function
TrpC5 functions as a sensor for extracellular stimuli to regulate intracellular Ca2+ levels.
Cell Type
Endothelial cells, Neurons
Biology Area
Cell Biology, Neuroscience
Molecular Family
Ion Channels
Antigen References
  1. Song K, et al. 2021. Elife. 10:e63429.
  2. Du SL, et al. 2021. Rev Cardiovasc Med. 22:127-135.
  3. Duan J, et al. 2019. Sci Adv. 5:eaaw7935.
Gene ID
7224 View all products for this Gene ID
UniProt
View information about TrpC5 on UniProt.org
Go To Top Version: 1    Revision Date: 10/18/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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