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Purified anti-Tudor-SN Antibody

Pricing & Availability
Clone
13.14.1 (See other available formats)
Regulatory Status
RUO
Other Names
SND1, TDRD11, Staphylococcal nuclease domain-containing protein 1, 100 kD coactivator, p100
Isotype
Mouse IgG1, κ
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Product Citations
publications
13141_Purified_Tudor-SN_Antibody_050218
Total lysates (15 µg protein) from 293T, 293T/Tudor-SN CRISPR/Cas9 knockout (KO) and 293T/Tudor-SN knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Tudor-SN antibody, clone 13.14.1 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 13141_Purified_Tudor-SN_Antibody_050218
    Total lysates (15 µg protein) from 293T, 293T/Tudor-SN CRISPR/Cas9 knockout (KO) and 293T/Tudor-SN knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Tudor-SN antibody, clone 13.14.1 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
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690002 100 µg $229
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Description

Tudor-SN, also called SND1 or p100, is a multifunctional protein. It has been identified in mammals, fish, Drosophila, Caenorhabditis elegans, ciliates and fission yeast, but not in bacteria. It was first characterized as a co-activator of Epstein-Barr virus nuclear protein 2, and subsequently found to interact with other transcription factors such as c-Myb, Pim-1, STAT6, and STAT5. Tudor-SN regulates pre-mRNA splicing through interacting with components of the spliceosome. Many studies also demonstrate that Tudor-SN potentially participates in the cell cycle regulation.

Studies have illustrated the significant association of Tudor-SN with different types of cancers, such as liver, breast, prostate and colorectal cancers and malignant glioma. It is overexpressed in these cancers and known to promote carcinogenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C-term region (698-910 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Ota H, et al. 2013. Cell. 153:575.
RRID
AB_2629792 (BioLegend Cat. No. 690002)

Antigen Details

Structure
910 amino acid with a predicted molecular weight of 102kD, but can be observed at ~110 kD due to post transitional modifications.
Distribution

Cytoplasm, nucleus and melanosome.

Function
Bridging factor between STAT6 and the basal transcription factor; PIM1 regulation of MYB activity; transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2.
Interaction
EBNA2, EAV NSP1, GTF2E1, GTF2E2, STAT6, POLR2A and STAT5.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Li CL, et al. 2008. Nucleic Acids Res. 36:3579.
2. Caudy AA, et al. 2003. Nature. 425:411.
3. Howard-Till RA, et al. 2007. Cell. 6:1795.
4. Tong X, et al. 1995. Mol. Cell. Biol. 15:4735.
5. Su C, et al. 2015. J. Biol. Chem. 290:7208.
6. Leverson JD, et al. 1998. Mol. Cell. 2:417.
7. Yang J, et al. 2002. EMBO J. 21:4950.
8. Jariwala N, et al. 2015. Int. J. Oncol. 46:465.

Gene ID
27044 View all products for this Gene ID
UniProt
View information about Tudor-SN on UniProt.org
Go To Top Version: 2    Revision Date: 05/02/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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