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- Clone
- A18118A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Chap1, DSK2 homolog, Protein linking IAP with cytoskeleton 2, PLIC-2, hPLIC-2, Ubiquitin-like product Chap1/Dsk2
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-Ubiquilin-2 antibody (clone A18118A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Ubiquilin-2 antibody (clone A18118A) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Ubiquilin-2 antibody (clone A18118A) on formalin-fixed paraffin-embedded human glioblastoma tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 867901 | 25 µg | $106 | ||||
| 867902 | 100 µg | $276 | ||||
Ubiqulin-2 is a ubiquitin-like protein that facilitates the degradation of ubiquitinated proteins by mediating their binding to the proteasome. Mutations in ubiquilin-2 have been linked to neurodegenerative diseases such as amyotrophic lateral sclerosis and frontal temporal dementia. These mutations are linked to disruption and impairment in the delivery of ubiquitinated substrates to the proteasome. In addition, ubiquilin-2 can interact with components of the ERAD machinery and localizes to autophagosomes, indicating that it plays a role in these pathways.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ubqln2 recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2810807 (BioLegend Cat. No. 867901)
AB_2810807 (BioLegend Cat. No. 867902)
Antigen Details
- Structure
- Human Ubiquilin-2 is a 624 amino acid protein with a predicted molecular mass of 65 kD and observed molecular mass of ~75 kD.
- Distribution
-
Tissue distribution: Widely expressed, highest expression in cerebellar vermis
Cellular distribution: Nucleus, cytoplasm, membrane, and autophagosome - Function
- Ubiquilin-2 plays an important role in the regulation of different protein degradation pathways including the ubiquitin-proteasome system, autophagy, and the endoplasmic reticulum-associated protein degradation.
- Interaction
- Ubiquilin-2 interacts with UBQLN1, UBE3A, BTRC, 19S proteasome subunit, C9orf72, HNRNPA1, and HNRNPU.
- Cell Type
- Neurons
- Biology Area
- Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- Autophagosome Markers
- Antigen References
-
- Deng HX. et al. 2011. Nature. 477(7363):211-5.
- Hjerpe R. et al. 2016. Cell. 166(4):935-949.
- Wu Q. et al. 2015. Acta Neuropathol. 129(3): 417–428.
- Gene ID
- 29978 View all products for this Gene ID
- UniProt
- View information about Ubiquilin-2 on UniProt.org
Related FAQs
Other Formats
View All Ubiquilin-2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Ubiquilin-2 | A18118A | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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