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Purified anti-VAV Antibody

Pricing & Availability
Clone
P82E11C10 (See other available formats)
Regulatory Status
RUO
Other Names
Vav Guanine Nucleotide Exchange Factor 1, Vav 1 Guanine Nucleotide Exchange Factor, Proto-Oncogene Vav, Vav 1 Oncogene
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P82E11C10_PURE_VAV_Antibody_1_010820.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-VAV antibody (clone P82E11C10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • P82E11C10_PURE_VAV_Antibody_1_010820.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-VAV antibody (clone P82E11C10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • P82E11C10_PURE_VAV_Antibody_2_010820.png
    Jurkat cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1.0 µg/mL (1:500 dilution) of either purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400102, panel A) or purified anti-VAV antibody (clone P82E11C10, panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • P82E11C10_PURE_VAV_Antibody_3_010820.png
    Whole cell extracts (250 μg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.0 µg of purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400102) or purified anti-VAV antibody (clone P82E11C10). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of VAV. Lane M: Molecular weight marker.
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935101 25 µg $101
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935102 100 µg $253
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Description

VAV1 is one of the three members of the VAV family that functions as a guanine exchange factor (GEF) and is exclusively expressed in hematopoietic and trophoblast cells. VAV1 plays a critical role in immune synapse formation through the regulation of actin cytoskeleton organization. Upon TCR activation, VAV1 is recruited to the immunological synapse and interacts via its SH2 and C-terminal SH3 domains with phosphorylated adaptor protein SLP-76. VAV1 gets phosphorylated and undergoes conformational change to expose its DH domain that modulates GEF activity and promotes GDP to GTP exchange of Rho GTPase CDC42. CDC42 activates WASP and leads to the activation of actin polymerization. In addition, VAV1 transduces T cell receptor signaling including NFAT and NF-κB pathways in a GEF independent manner and promotes T cell activation and development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human VAV1 (amino acid 121-324)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Direct ELISA, FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize mouse VAV1.

Both methanol and Triton X-100 permeabilization produced correct staining in ICC testing.

RRID
AB_2832888 (BioLegend Cat. No. 935101)
AB_2832888 (BioLegend Cat. No. 935102)

Antigen Details

Structure
VAV1 is a 845 amino acid protein with a predicted molecular weight of 98 kD.
Distribution

Cytosol/Hematopoeitic cells

Function
GEF, TCR signal transduction
Biology Area
Cell Biology
Antigen References
  1. Costello P. et al. 1999. Proc Natl Acad Sci USA. 96:3035.
  2. Wülfing C. et al. 2000. Proc Natl Acad Sci USA. 97:10150.
  3. Villalba M, et al. 2001. J. Cell. Biol. 155:331.
  4. Tybulewicz V, et al. 2003. Immunological Reviews. 192:42.
  5. Katzav S. et al. 2004. Blood. 103:2443.
  6. Fernandez-Zapico M. et al. 2005. Cancer. Cell. 7:39.
Gene ID
7409 View all products for this Gene ID
UniProt
View information about VAV on UniProt.org

Other Formats

View All VAV Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-VAV P82E11C10 WB,ICC,IP,Direct ELISA
Go To Top Version: 2    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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