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Purified anti-VWF Antibody

Pricing & Availability
Clone
A21125D/G (See other available formats)
Regulatory Status
RUO
Other Names
von Willebrand factor (vWF), F8VWD, VWD, Coagulation factor VIII antibody, Coagulation factor VIII VWF antibody
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A21125DG_PURE_VWF_Antibody_1_011224
IHC staining of Purified anti-VWF (clone A21125D/G) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated with 5 µg/mL of Purified anti-VWF (clone A21125D/G) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 40X objective. Scale bar: 50 µm
  • A21125DG_PURE_VWF_Antibody_1_011224
    IHC staining of Purified anti-VWF (clone A21125D/G) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated with 5 µg/mL of Purified anti-VWF (clone A21125D/G) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 40X objective. Scale bar: 50 µm
  • A21125DG_PURE_VWF_Antibody_2_011224
    HeLa cells (negative cell line, panel A) and HUVEC cells (positive cell line, panel B) were treated with Brefeldin A (Cat. No. 420601) for 4 hours and fixed and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 10 µg/mL of Purified Mouse IgG2b, κ Isotype Control (clone MPC-11) (Cat. No. 400302) (panels, A-, B-) or 10 µg/mL of Purified anti-VWF (clone A21125D/G) (panels A+, B+), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 60X objective. Scale bar: 50 µm
  • A21125DG_PURE_VWF_Antibody_3_011224
    HUVEC cells (positive cell line, filled histogram) and HeLa cells (low-expressing cell line, open histogram, solid line) were treated with Brefeldin A (Cat. No. 420601) for 4 hours and fixed/permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-VWF (clone A21125D/G), or Purified Mouse IgG2b, κ Isotype Control (clone MPC-11) (Cat. No. 400302) (open histogram, dashed line).
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628851 25 µg $165
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628852 100 µg $415
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Description

Von Willebrand factor (VWF) plays a crucial role in blood clotting by assisting platelets in forming clots at the site of blood vessel injury. Acting as a bridge between platelets and the damaged blood vessel walls, VWF facilitates platelet adhesion and aggregation, which are essential steps in the clotting process. Moreover, VWF also stabilizes factor VIII, a key protein in the blood clotting cascade, preventing its degradation and enhancing its availability for clot formation. Mutations or deficiencies in VWF results in defects in platelet adhesion and potentially bleeding disorders such as thrombotic thrombocytopenic purpura, Heyde’s syndrome, and von Willebrand disease. A critical region of VWF is the A2 domain. Contained in this domain is the cleavage site for the ADAMTS13 protease which inactivates VWF.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant portion of human VWF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1 - 10 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone A21125D/G) is not suitable for use in western blot.

This antibody (clone A21125D/G) is a pool of two monoclonal antibodies, A21125D and A21125G.

For intracellular flow cytometry (ICFC), fixation/permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401) is recommended.

RRID
AB_3097540 (BioLegend Cat. No. 628851)
AB_3097540 (BioLegend Cat. No. 628852)

Antigen Details

Structure
VWF is a 2,813 amino acid protein with a predicted molecular weight of 309 kD.
Distribution

ECM, secreted, cytoplasmic

Function
Structural protein, multimeric
Interaction
Factor VIII (F8), ADAMTS13, GPIb receptor, heparin, collagen, platelet integrin αIIbβ3
Cell Type
Platelets
Antigen References
  1. Randi AM, et al. 2018. Blood. 132:132-40.
  2. Xiang Y & Hwa J. 2016. Curr Opin Hematol. 23:288-93.
  3. Chen J & Chung DW. 2018. Blood. 132:141-147.
  4. Lynch CJ, et al. 2014. Blood. 123:2585-92.
Gene ID
7450 View all products for this Gene ID
UniProt
View information about VWF on UniProt.org

Other Formats

View All VWF Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-VWF A21125D/G IHC-P,ICC,ICFC
Go To Top Version: 1    Revision Date: 01/12/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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