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Purified anti-Wnt7a Antibody

Pricing & Availability
Clone
W19053A (See other available formats)
Regulatory Status
RUO
Other Names
Wingless-Type MMTV Integration Site Family, Member 7A (WNT7A), Wnt Family Member 7A, Proto-Oncogene Wnt7a Protein, Protein Wnt7a
Isotype
Rat IgG2a, κ
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Product Citations
publications
19053A_PURE_Wnt7a_Antibody_2_011122
IHC staining of purified anti-Wnt7a antibody (clone W19053A) on formalin-fixed paraffin-embedded mouse brain tissue. The tissue was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 594 goat anti-rat IgG antibody (yellow) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20x objective.
  • 19053A_PURE_Wnt7a_Antibody_2_011122
    IHC staining of purified anti-Wnt7a antibody (clone W19053A) on formalin-fixed paraffin-embedded mouse brain tissue. The tissue was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 594 goat anti-rat IgG antibody (yellow) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20x objective.
  • W19053A_PURE_Wnt7a_Antibody_1_011122
    ICC staining of purified anti-Wnt7a antibody (clone W19053A) and NIH-3T3 cells were resolved with 4% PFA, and permeabilized with 100% ice-cold methanol. Cells were then blocked with 5% fetal bovine serum in PBS for 30 minutes, and then incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG antibody (red) (Cat. No. 405422) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective.
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949501 25 µg $118
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949502 100 µg $293
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Description

The WNT protein family has a highly conserved cysteine-rich structure that associates with the extracellular matrix (ECM) with high affinity through binding to heparin sulfate glycoproteins. They are carried intercellularly by lipoproteins and mediate signaling activity by binding to transmembrane Frizzled receptors and low-density-related proteins (LRP). They elicit a variety of responses through canonical or non-canonical (PCP or Ca2+) pathways. There are two subtypes of WNT7 that have been identified, WNT7a and WNT7b.

WNT7a is a secreted cell-surface signaling protein that controls a set of pathways that act on cell fate determination and tissue patterning during embryogenesis. It is known that WNT7a can modulate the development of the uterus, cerebellum and limbs, while WNT7b is important in lung and placental development. The human amino acid sequence of WNT7a shares high similarity (99.4%) with mouse WNT7a. Signals transduced through WNT7a and its receptor are critical for female organ development, maintaining levels of female sex hormones and the development of oviduct and uterus. Receptors for WNT7a include frizzled-5, frizzled-7 and frizzled-10. WNT7a binds to frizzled-7 to induce signaling through the SKT/mTOR pathway in order to further modulate myofibers in a G-protein dependent PI3K pathway.

During muscle cell regeneration, WNT7a in the ECM along with transiently raised fibronectin levels in the stem cell niche can bind the frizzled-7/Sdc4 receptor complex and synergistically induce symmetric divisions of satellite stem cells. Also, in neurogenesis and chondrogenesis, WNT7a contributes to regulating and maintaining the level of lineage differentiation factors required to promote apoptosis/cell death through the binding of different receptors and interactions with ECM proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant mouse Wnt7a protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC fix/perm, use 100% methanol or 4% PFA followed by 100% methanol. It is not recommended for western blotting. Cross-reactivity versus other Wnt family members were not tested.

RRID
AB_2910517 (BioLegend Cat. No. 949501)
AB_2910517 (BioLegend Cat. No. 949502)

Antigen Details

Structure
Wnt7a is 349 amino acid protein with a predicted molecular mass of ~39 kD.
Distribution

WNT7a is expressed in the placenta, kidney, testis, uterus, fetal lung, fetal and adult brain.

Function
WNT7a is important for dorsal ectoderm tissue development and regulation.
Interaction
Binding of Wnt7a with Frizzled receptors (Fzd5, Fzd7, Fzd10) induces activation of Disheveled 1 (Dvl1), which stimulates downstream effector pathways mediated by e.g. the small GTPases RhoA and Rac1, along with CamKII.
Ligand/Receptor
Frizzled receptors, LRP co-receptors.
Molecular Family
Growth Factors, Neurotrophic Factors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
22421 View all products for this Gene ID
UniProt
View information about Wnt7a on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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