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Purified Mouse IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG2b-57 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
MG2b-57_GoChIP_IgG2b-k_ctrl_1_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MG2b-57, Cat. No. 401213) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG2b-57_GoChIP_IgG2b-k_ctrl_1_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MG2b-57, Cat. No. 401213) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG2b-57_GoChIP_IgG2b-k_ctrl_2_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone MG2b-57, Cat. No. 401213) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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401201 50 µg $47
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Description

The isotype of MG2b-57 immunoglobulin is mouse IgG2b,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested
ChIP - Verified
ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular flow cytometry, Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, functional assay. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 401215-401220) as negative control.

Application References

(PubMed link indicates BioLegend citation)
  1. Magatti M, et al. 2008. Stem Cells 26:182. (FC) PubMed
Product Citations
  1. Hutt M, et al. 2018. Oncotarget. 9:11322. PubMed
  2. Wallis J, et al. 2020. Eur J Pharm Sci. 152:105456. PubMed
  3. Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed
  4. Tsoi LC, et al. 2020. JCI Insight. 5:00. PubMed
  5. Ishikawa R, et al. 2022. FEBS Open Bio. 12:1178. PubMed
  6. Peláez R, et al. 2017. PLoS One.. 10.1371/journal.pone.0181579. PubMed
  7. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  8. Minnella AM, et al. 2018. Sci Rep. 8:17522. PubMed
  9. Lu F, et al. 2018. Exp Neurol. 299:65. PubMed
  10. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  11. Yen YT, et al. 2021. Nat Commun. 12:7297. PubMed
RRID
AB_2744505 (BioLegend Cat. No. 401201)
AB_2744505 (BioLegend Cat. No. 401202)

Antigen Details

Gene ID
NA

Other Formats

View All Reagents
Description Clone Applications
Purified Mouse IgG2b, κ Isotype Ctrl MG2b-57 FC,ICFC,WB,IP,ICC,IHC,FA,ChIP
Biotin Mouse IgG2b, κ Isotype Ctrl MG2b-57 FC,ICFC
FITC Mouse IgG2b, κ Isotype Ctrl MG2b-57 FC,ICFC
PE Mouse IgG2b, κ Isotype Ctrl MG2b-57 FC,ICFC
APC Mouse IgG2b, κ Isotype Ctrl MG2b-57 FC,ICFC
Ultra-LEAF™ Purified Mouse IgG2b, κ Isotype Ctrl MG2b-57 FC,ICFC,WB,IP,ICC,IHC,FA
Go To Top Version: 2    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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