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Purified Mouse IgG3, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG3-35 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG3, κ
Ave. Rating
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Product Citations
publications
MG3-35_GoChIP_IgG3_Iso-Con_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) (Clone 1B1-B2, Cat. No. 819403) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG3, κ Isotype Control Antibody (Clone MG3-35, Cat. No. 401325) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG3-35_GoChIP_IgG3_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-Histone H3 (C-terminus) (Clone 1B1-B2, Cat. No. 819403) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG3, κ Isotype Control Antibody (Clone MG3-35, Cat. No. 401325) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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401301 50 µg $47
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401302 500 µg $129
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Description

The isotype of MG3-35 immunoglobulin is mouse IgG3,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested
ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

The MG3-35 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401310) as negative control.

Product Citations
  1. Silva MA, et al. 2018. PLoS One. 13:e0196464. PubMed
  2. Wallis J, et al. 2020. Eur J Pharm Sci. 152:105456. PubMed
  3. Rolle A, et al. 2016. Proc Natl Acad Sci U S A. 10.1073/pnas.1518836113. PubMed
  4. Pleiner T, et al. 2018. J Cell Biol. 217:1143. PubMed
RRID
AB_2889135 (BioLegend Cat. No. 401301)
AB_2889135 (BioLegend Cat. No. 401302)

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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