RBC Lysis Buffer (10X)

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RUO
Other Names
Red blood cell lysis buffer
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420301 100 mL $65
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420302 500 mL $206
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Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical data sheet

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References

(PubMed link indicates BioLegend citation)
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  4. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  5. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  6. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  7. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  8. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  9. Melendez E, et al. 2022. Development. 149: . PubMed
  10. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  11. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  12. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  13. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  14. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  15. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  16. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  17. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  18. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  19. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  20. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  21. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  22. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  23. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  24. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  25. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  26. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  27. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  28. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  29. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  30. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  31. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  32. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  33. Feng J, et al. 2022. Immunity. 55:405. PubMed
  34. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  35. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  36. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  37. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  38. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  39. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  40. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  41. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  42. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  43. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  44. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  45. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  46. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  47. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  48. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  49. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  50. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  51. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  52. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  53. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  54. Le DT, et al. 2023. iScience. 26:106059. PubMed
  55. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  56. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  57. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  58. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  59. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  60. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  61. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  62. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  63. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  64. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  65. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  66. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  67. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  68. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  69. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  70. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  71. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  72. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  73. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  74. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  75. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  76. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  77. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  78. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  79. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  80. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  81. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  82. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  83. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  84. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  85. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  86. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  87. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  88. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  89. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  90. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  91. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  92. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  93. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  94. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  95. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  96. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  97. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  98. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  99. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  100. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  101. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  102. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  103. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  104. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  105. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  106. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  107. Meng S, et al. 2022. Exp Anim. . PubMed
  108. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  109. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  110. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  111. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  112. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  113. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  114. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  115. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  116. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  117. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  118. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  119. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  120. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  121. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  122. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  123. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  124. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  125. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  126. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  127. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  128. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  129. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  130. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  131. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  132. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  133. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  134. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  135. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  136. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  137. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  138. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  139. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  140. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  141. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  142. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  143. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  144. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  145. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  146. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  147. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  148. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  149. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  150. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  151. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  152. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  153. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  154. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  155. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  156. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  157. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  158. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  159. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  160. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  161. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  162. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  163. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  164. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  165. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  166. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  167. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  168. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  169. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  170. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  171. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  172. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  173. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  174. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  175. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  176. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  177. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  178. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  179. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  180. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  181. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  182. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  183. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  184. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  185. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  186. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  187. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  188. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  189. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  190. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  191. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  192. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  193. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  194. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  195. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  196. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  197. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  198. Sci T 2013. Toxicol Sci. 136:72. PubMed
  199. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  200. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  201. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  202. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  203. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  204. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  205. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  206. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  207. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  208. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  209. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  210. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  211. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  212. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  213. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  214. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  215. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  216. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  217. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  218. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  219. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  220. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  221. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  222. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  223. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  224. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  225. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  226. Zhao J, et al. 2013. Elife. 21:537. PubMed
  227. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  228. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  229. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  230. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  231. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  232. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  233. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  234. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  235. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  236. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  237. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  238. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  239. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  240. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  241. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  242. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  243. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  244. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  245. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  246. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  247. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  248. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  249. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  250. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  251. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  252. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  253. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  254. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  255. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  256. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  257. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  258. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  259. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  260. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  261. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  262. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  263. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  264. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  265. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  266. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  267. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  268. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  269. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  270. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  271. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  272. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  273. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  274. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  275. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  276. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  277. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  278. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  279. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  280. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  281. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  282. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  283. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  284. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  285. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  286. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  287. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  288. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  289. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  290. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  291. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  292. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  293. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  294. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  295. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  296. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  297. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  298. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  299. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  300. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  301. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  302. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  303. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  304. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  305. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  306. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  307. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  308. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  309. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  310. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  311. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  312. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  313. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  314. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  315. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  316. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  317. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  318. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  319. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  320. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  321. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  322. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  323. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  324. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  325. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  326. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  327. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  328. Trial J, et al. 2020. Geroscience. . PubMed
  329. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  330. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  331. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  332. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  333. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  334. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  335. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  336. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  337. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  338. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  339. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  340. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  341. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  342. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  343. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  344. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  345. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  346. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  347. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  348. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  349. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  350. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  351. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  352. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  353. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  354. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  355. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  356. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  357. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  358. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  359. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  360. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  361. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  362. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  363. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  364. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  365. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  366. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  367. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  368. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  369. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  370. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  371. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  372. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  373. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  374. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  375. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  376. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  377. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  378. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed
  379. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  380. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  381. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  382. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  383. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  384. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  385. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  386. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA
Go To Top Version: 3    Revision Date: 06/10/2022

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