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- Regulatory Status
- RUO
- Other Names
- Amyloid Beta A4 Protein, Prea4, Protease Nexin-II, Peptidase Nexin-II, Beta-Amyloid Peptide, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, APP, Amyloid Precursor Protein
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Covance Catalog# PTN-5279
- Ave. Rating
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- Product Citations
- publications
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Western blot of recombinant Human APP770. Lanes 1, 3, 5: Molecular weight marker; Lanes 2, 4, 6: 0.025 µg of recombinant human APP770 protein. The blots were incubated with 1 µg/ml of purified anti-APP, 573-596 antibody (clone 1G6, lanes 1 & 2, Cat. No. 806001), purified anti-β-Amyloid, 1-16 antibody (clone 6E10, lanes 3 &4, Cat. No. 803001) or purified anti-APP C-Terminal Fragment antibody (clone C1/6.1, lanes 5 & 6, Cat. No. 802803) overnight at 4°C. HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 843201 | 25 µg | $222 | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Human full-length APP recombinant protein (a.a. 1 - 770) with 10X His-tag at the N-terminus. The protein was expressed in HEK293 cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Purity
- >85% by SDS-PAGE
- Formulation
- 25 mM Tris-HCl, 150 mM NaCl, 0.1% dodecyl maltoside (DDM), pH 8.0.
- Concentration
- 0.1 mg/ml
- Storage & Handling
- Store frozen (-20 or -70°C); shipped in blue ice. Aliquot upon receipt before freezing. Avoid repeated freeze/thaw cycles.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid
- Gene ID
- 351 View all products for this Gene ID
- UniProt
- View information about APP770 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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