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- Regulatory Status
- RUO
- Other Names
- IBP5, IGFBP5, IGF-binding protein 5, insulin-like growth factor binding protein 5, insulin-like growth factor-binding protein 5, IGF-binding protein IBP-5, IGFBP-5
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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When recombinant human IGFBP-5 is immobilized at 0.5 μg/mL, biotinylated recombinant human IGF-II binds in a dose-dependent manner with ED50 range of 0.9 – 5.4 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding. -
Stability Testing for Recombinant Human IGFBP-5. Human IGFBP-5 was aliquoted in 40 mM sodium citrate, pH4 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4⁰C (Control). The samples were tested in a binding assay with biotinylated recombinant human IGF-II. The ED50 for this effect is 0.9 – 5.4 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 797804 | 25 µg | $315 | ||||
| 797806 | 100 µg | $741 | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Seven IGFBPs have been described to modulate the IGF activity. IGFBPs transport IGFs, in which they may inhibit mitogenesis, differentiation, survival, and other IGF-stimulated events. IGFBPs structurally are characterized by three domains: the amino-terminal, the carboxi-terminal, and an intermedia or variable L-domain. Members of the IGFBP family exhibit 67–70% structural homology. The greatest homology among the IGFBPs is in the N- and C-terminal regions. Some IGFBPs bind to the extracellular matrix (IGFBP-2, -3, -5, and -6). In fact, a heparin-binding domain (HBD) has been identified in the C-terminal region of these binding proteins. IGFBP-5 is the most conserved IGFBP in mammals. Additionally, human and mouse IGFBP-5 are 97% identical. The IGFBPs bind IGFs with high affinity (kD ∼0.1 nM), and this binding restricts the interaction of IGFs to their receptors. The release of the IGFs from IGFBP-5 is mediated by proteolysis; it has been described that IGFBP-5 is cleaved by ADAM-12, ADAM-9, C1s, and PAPPs (pregnancy-associated plasma proteases) A and A2. IGFBP-5 plays a key role in tissue repair and regeneration. IGFBP-5 is overexpressed in fibrotic lung tissues from patients with idiopathic pulmonary fibrosis. In vitro, IGFBP-5 induces the production of ECM components while in vivo, IGFBP-5 initiates the development of dermal and pulmonary fibrosis. In addition, IGFBP-5 induces lung fibroblast and mononuclear cell migration in an IGF-I–independent manner. IGFBP-5 plays an important role in mammary development, involution, and breast cancer metastasis. IGFBP-5 has nuclear localization sequence that mediates β-importin-dependent translocation to the nucleus, where it interacts with the vitamin D receptor and modulates the vitamin D response in osteoblasts.
Product DetailsProduct Details
- Source
- Human IGFBP-5, amino acids (Leu21 -Glu272) (Accession# P24593.1), was expressed in 293E cells.
- Molecular Mass
- The 252 amino acid recombinant protein has a predicted molecular mass of approximately 28.57 kD. The DTT-reduced and non-reduced protein migrates at approximately 31-35 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 40mM sodium citrate, pH4.
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When recombinant human IGFBP-5 is immobilized at 0.5 μg/mL, biotinylated recombinant human IGF-II binds in a dose-dependent manner with ED50 range of 0.9 – 5.4 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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IGFBP-5 is expressed in a big range of cell types and tissues.
- Function
- IGFBP-5 regulates the activity of both IGF-I and IGF-II by preventing their binding to the receptors. IGFBP-5 regulates osteogenesis, muscle differentiation, and kidney development, among other processes.
- Interaction
- Variety of cells
- Ligand/Receptor
- IGF-I, IGF-II, and nuclear vitamin D receptor (VDR)
- Bioactivity
- Biotinylated recombinant human IGF-II binds to immobilized recombinant human IGFBP-5 in a dose-dependent manner
- Cell Type
- Epithelial cells, Fibroblasts, Osteoblasts
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability
- Molecular Family
- Growth Factors
- Antigen References
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- Schuller AG, et al. 1994. Mol Cell Endocrinol. 104:57-66.
- Overgaard MT, et al. 2001. J Biol Chem. 276:21849-53.
- Mohan S, et al. 2002. Biochemistry. 41:15394-403.
- Beattie J, et al. 2006. Biochem J. 395:1-19.
- Schedlich LJ, et al. 2007. Mol Endocrinol. 21:2378-90.
- Akkiprik M, et al. 2008. Breast Cancer Res. 10:212.
- Yasuoka H, et al. 2009. Am J Respir Cell Mol Biol. 41:179-88.
- Nili M, et al. 2012. J Biol Chem. 287:1510-9.
- Gene ID
- 3488 View all products for this Gene ID
- UniProt
- View information about IGFBP-5 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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