Recombinant Human IL-6 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
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Product Citations
publications
Human_IL-6_CF_RECOM_ComparisonChart_041919.png
Recombinant human IL-6 induces the proliferation of 7TD1 cells in a dose-dependent manner. BioLegend's product was compared side-by-side to the leading competitor equivalent product.
  • Human_IL-6_CF_RECOM_ComparisonChart_041919.png
    Recombinant human IL-6 induces the proliferation of 7TD1 cells in a dose-dependent manner. BioLegend's product was compared side-by-side to the leading competitor equivalent product.
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570802 10 µg $147
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570804 25 µg $253
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570806 100 µg $698
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570808 500 µg $1758
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Description

IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.

Product Details
Technical data sheet

Product Details

Source
Human IL-6, amino acids Pro29-Met212 (Accession # NM_000600) was expressed in E. coli.
Molecular Mass
The 185 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21,041 Da. The DTT-reduced and non-reduced protein migrate at approximately 23kDa by SDS-PAGE.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
PBS, pH 7.2, 0.5M NaCl, 10% Glycerol
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is 4-20 pg/ml, corresponding to a specific activity of 0.5-2.5 X108 units/mg, as determined by a dose dependent stimulation in a 7TD1 cell proliferation assay.

The specific activity of recombinant human IL-6 is approximately 5.0 x 104 IU/µg when compared against the 1st WHO International Standard for Interleukin-6 (NIBSC code: 89/548) as determined by the dose dependent stimulation of 7TD1 cell proliferation.

For more information on specific activity, please visit the Recombinant Protein Unit Conversions page.
Application

Bioassay

Recommended Usage

Use when high specific biological activity is required.

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Reitsma JM, et al. 2013. J Virol. 87:10763. PubMed
Product Citations
  1. Khanam A, et al. 2022. Hepatology. 75:690. PubMed
  2. Park SA, et al. 2023. Int J Mol Sci. 24:. PubMed
  3. Woodley K, et al. 2023. Nat Commun. 14:2132. PubMed
  4. Devi S, et al. 2023. Cell Rep. 112265:42. PubMed
  5. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  6. Kaiser R, et al. 2021. JCI Insight. 6:e150862. PubMed
  7. Reitsma J, et al. 2013. J Virol. 87:10763. PubMed
  8. Zhang H, et al. 2020. Front Cell Dev Biol. 8:205. PubMed
  9. Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed
  10. Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed
  11. Gopinath A, et al. 2021. NPJ Parkinsons Dis. 7:62. PubMed
  12. Yamaguchi Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  13. Fulciniti M, et al. 2018. Cell Rep. 25:3693. PubMed
  14. Ibrahim ML, et al. 2018. Cell Rep. 25:3036. PubMed
  15. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  16. Miyamoto T, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  17. Guo Q, et al. 2022. Blood Adv. 6:5668. PubMed
  18. Wong GK, et al. 2019. JCI Insight. 4. PubMed
  19. Ianniciello A, et al. 2021. Sci Transl Med. 13:eabd5016. PubMed
  20. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
  21. Jokela TA, et al. 2018. Front Cell Dev Biol. 6:41. PubMed

Antigen Details

Structure
Neuropoietic cytokine family
Distribution

T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells

Function
Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Interaction
T cells, B cells, hepatocytes, cholinergic neurons
Ligand/Receptor
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130, gp130)
Bioactivity
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.

Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 6    Revision Date: 01/13/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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