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- Regulatory Status
- RUO
- Other Names
- SELL, CD62 antigen-like family member L, CD62L, Leukocyte-endothelial cell adhesion molecule 1 (LECAM1), Leukocyte adhesion molecule 1 (LAM-1), Leukocyte surface antigen Leu-8, Lymph node homing receptor, TQ1, gp90-MEL, Lymphocyte antigen 22 (Ly-22)
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- Submit a Review
- Product Citations
- publications
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Immobilized recombinant human L-Selectin induces adhesion of U937 cells in a dose dependent manner. ED50 for this effect is 0.5 - 2.0 µg/mL. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells. -
Stability testing for human L-Selectin. Human L-Selectin was aliquoted in PBS, pH 7.2 at 0.2 mg/ml. One aliquot was freeze and thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested in U937 cell adhesion assay.
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L-selectin, belonging to the selectin/LECAM family, is a type I transmembrane protein, consisted of one C-type lectin domain and EGF-like repeats, followed by a transmembrane region and a short cytoplasmic tail of 17 amino acids. L-selectin gene encodes a protein with a predicted size of 42 kD, but the actual molecular weight is around 70 kD to 120 kD, due to N-linked glycosylation. L-selectin is constitutively expressed on leukocytes. As a homing receptor, L-selectin mediates adhesion and rolling of leukocytes on endothelial cells, in turn leukocytes enter peripheral lymphoid organs via high endothelial venules (HEV). L-selectin also directs leukocytes to inflammation sites. Circulating leukocytes can also tether on adherent leukocytes through the interaction between L-selectin and P-selectin glycoprotein ligand-1 (PSGL-1). L-selectin also interacts with mucins, such as CD34, glycosylated cell adhesion molecule-1 (GlyCAM-1) and podocalyxin. Ligand-induced clustering of L-selectin enhances leukocyte tethering and causes signaling cascade, promoting activation of integrin and mobilizing chemokine receptor on cell surface. To negatively regulate leukocyte recruitment and migration, L-selectin is cleaved by ADAM17, released from cell surface (ectodomain shedding) with functional activity. High level of soluble L-selectin can be detected in human plasma.
Product DetailsProduct Details
- Source
- Human L-Selectin, amino acids (Trp39 - Asn332) (Accession#: P14151), with a C-terminal human IgG1 Fc and 6xHis tag, was expressed in 293E cells.
- Molecular Mass
- The 535 amino acid recombinant protein has a predicted molecular mass of approximately 60.19 kD. The DTT-reduced and non-reduced protein migrates at approximately 75 and 150 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Trp.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10-25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes and larger are bottled at the concentration indicated on the vial.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50 = 0.5 - 2 µg/mL as measured by the ability of immobilized protein to support the adhesion of U937 cells. Calcein-AM (Cat. No. 425201) was used to measure the number of adherent cells.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Adhesion molecule
- Distribution
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Lymphoid tissues, leukocytes, cell surface and plasma.
- Function
- L-Selectin mediates leukocyte rolling and homing via HEV.
- Interaction
- Endothelial cells of high endothelial venules (HEVs), activated endothelial cells, and leukocytes.
- Ligand/Receptor
- P-selectin glycoprotein ligand-1 (PSGL-1), PODXL2, and mucins, such as CD34, GlyCAM-1, podocalyxin.
- Bioactivity
- Supports adhesion of U937 cells.
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- McEver RP. 2002. Curr. Opin. Cell Biol. 14:581-6.
- Rosen SD. 2004. Annu. Rev. Immunol. 22:129-56.
- Giblin PA, et al. 1997. J. Immunol. 159:3498-507.
- Ding Z, et al. 2003. Blood 101:4245-52.
- Dwir O, et al. 2002. J. Biol. Chem. 277:21130-9.
- Martinez M, et al. 2004. J. Biol. Chem. 280:5378-90.
- Ridger VC, et al. 2005. Am. J. Pathol. 166:945-52.
- Lasky LA, et al. 1992. Cell. 69:927-38.
- Puri KD, et al. 1995. J. Cell. Biol. 131:261-70.
- Kerr SC, et al. 2008. J. Immunol. 181:1480-90.
- Rosen SD. 2004. Annu. Rev. Immunol. 22:129-56.
- Schleiffenbaum B, et al. 1992. J. Cell. Biol. 119:229-38.
- Li Y, et al. 2006. Blood. 108:2275-9.
- Gene ID
- 6402 View all products for this Gene ID
- UniProt
- View information about L-Selectin on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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