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Recombinant Human TNF-α (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2,
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Product Citations
publications
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570109 4 pack $94
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Description

TNF-α is released from macrophages, monocytes, neutrophils, T-cells (principally CD4+), NK-cells and many transformed cell lines. Soluble homotrimeric TNF-α is released from cells by proteolysis of the integral membrane precursor form of TNF-α. TNF-α binding to some TNF-α receptors induces apoptosis and depending on cell type, receptor expression, and signal transduction status can induce other responses. TNF-α is involved in the inflammatory response.

Product Details
Technical data sheet

Product Details

Source
Human TNF-alpha, amino acids Val77-Leu233 (Accession # HUMTNFAB) was expressed in E. coli.
Molecular Mass
The 157 amino acid recombinant protein has a predicted molecular mass of 17,352 Da. The DTT-reduced and non-reduced protein migrates at approximately 16kDa by SDS-PAGE. The N-terminal amino acid is Val.
Purity
>98%, as determined by SDS-PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level
Less than 0.01ng per µg cytokine as determined by the LAL method.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application

ELISA
Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA: This TNF-a protein is useful as a standard for a human TNF-a sandwich ELISA, using unlabeled MAb1 antibody (Cat. No. 502802) for capture and biotinylated MAb11 antibody (Cat. No. 502904) for detection.

Product Citations
  1. Biesmans S, et al. 2015. Biomed Res Int. 716920:2015. PubMed
  2. Hübner M, et al. 2020. Cancers (Basel). :12. PubMed
  3. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed

Antigen Details

Structure
TNF superfamily; homotrimer
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Reconstitution
Sterile phosphate-buffered saline is recommended.
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Regulation
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Gene ID
7124 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org
Go To Top Version: 2    Revision Date: 02/22/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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