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- Regulatory Status
- RUO
- Other Names
- Growth Differentiation Factor 6, Bone Morphogenetic Protein 13, BMP13, BMP-13, GDF6, KFS1, CDMP-2, cartilage-derived morphogenetic protein
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- Product Citations
- publications
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Recombinant mouse GDF-6 induces alkaline phosphatase production in the chondrogenic cell line ATDC5. The ED50 for this effect is 0.5 – 2.5 µg/mL. -
Stability Testing for Recombinant Mouse GDF-6. Recombinant mouse GDF-6 was aliquoted in 0.1% TFA (trifluoroacetic acid). One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4⁰C (Control). The samples were tested for their ability to induce alkaline phosphatase production in the chondrogenic cell line ATDC5. The ED50 for this effect is 0.5 – 2.5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 795304 | 25 µg | $253 | ||||
| 795306 | 100 µg | $698 | ||||
Growth Differentiation Factor 6 (GDF-6, also known as BMP-13) is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptors, and activin receptor-like kinase I. Three type II receptors have also been recognized: type II BMP receptors and type II and IIB activin receptors. GDF-6 has high degree of homology (82%) to GDF-5 and GDF-7, so they define a subgroup within the BMP family. The mature form of mouse GDF-6 is a 120 amino acid homodimeric protein containing the characteristic seven conserved cysteine residues. GDF-6 was initially identified in bovine cartilage. De novo implantation of GDF-6 induces neo-tendon/ligament formation in rats in connective tissue healing experiments. Mutation in the GDF6 gene is associated with Klippel Feil syndrome, characterized by congenital fusion of the cervical spine vertebrae, and causes defects in joint, ligament, and cartilage formation in a transgenic mouse model. Bone marrow multipotent mesenchymal stromal cells (BM MSCs) are capable of differentiating into multiple cell types including osteoblasts and chondrocytes. Using BM MSCs, it was identified that GDF-6 inhibits osteogenic differentiation, and therefore deceleration of calcium mineralization and reduction in alkaline phosphatase induction. GDF-6 derived from young MSCs reduces age-associated functional decline in multiple tissues.
Product DetailsProduct Details
- Source
- Mouse GDF-6, amino acid (Thr335-Arg454) (Accession: # P43028), was expressed in E.coli.
- Molecular Mass
- The 121 amino acid recombinant protein has a predicted molecular mass of approximately 13.7 kD. The DTT-reduced and non-reduced protein migrates at approximately 15 and 28 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Thr.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in 0.1% TFA.
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2ᵒC and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant mouse GDF-6 induces alkaline phosphatase production in the chondrogenic cell line ATDC5. The ED50 for this effect is 0.5 – 2.5 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer
- Distribution
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MSCs, mesenchymal cells, neurons
- Function
- Regulates ectoderm patterning, eye development, bone formation, normal formation of axial and appendicular skeleton (bones and joints in the limbs).
- Interaction
- Bone marrow multipotent mesenchymal stromal cells, chondroblasts
- Ligand/Receptor
- BMPR1A, BMPR1B, BMPR2 and ACVR2A
- Bioactivity
- Recombinant mouse GDF-6 induces alkaline phosphatase production in the chondrogenic cell line ATDC5.
- Cell Type
- Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
- Biology Area
- Cardiovascular Biology, Cell Biology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Growth Factors
- Antigen References
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1. Storm EE, et al., 1994, Nature 368 (6472):639.
2. Williams LA, et al., 2008, Int J Biol Sci. 4:318.
3. Chen D, et al. 2004. Growth Factors. 22:233.
4. Williams LA, et al. 2008. Int. J. Biol. Sci. 4:318.
5. Tassabehji M, et al. 2008. Hum. Mutat. 29:1017.
6. Shen B, et al. 2009. Int. J. Biol. Sci. 5:192.
7. Hisamatsu D, et al., 2016. Aging 8:1259.
8. El Bialy I, et al., 2017. Pharm Res. 34:1152. - Gene ID
- 242316 View all products for this Gene ID
- UniProt
- View information about GDF-6 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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