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- Regulatory Status
- RUO
- Other Names
- AMHR, AMHR2, MRII, MISRII, MISR2
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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When recombinant human AMH is immobilized at 1 µg/mL, recombinant rat AMHR2/MIS RII binds in a dose-dependent manner with ED50 of 0.25 – 1.5 µg/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding. -
Stability Testing for Recombinant Rat AMHR2. Recombinant Rat AMHR2/MIS RII was aliquoted in PBS. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with recombinant human AMH. The ED50 for this effect is 0.25 – 1.5 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 565704 | 25 µg | $320 | ||||
| 565706 | 100 µg | $645 | ||||
Anti-Müllerian Hormone receptor type II (AMHR2) is the receptor for Anti-Müllerian Hormone (AMH). AMH, also known as Müllerian Inhibiting substance (MIS), belongs to transforming growth factor beta (TGF-β) family. AMH activates downstream serine/ threonine kinase activity by recruiting type I and type II receptors. AMH uniquely possesses AMHR2. Upon AMH-AMHR2 binding, AMHR2 is phosphorylated and activates non-ligand binding kinase domain of type I receptor (ALK2, ALK3 and ALK6). This leads to the translocation of SMAD heterodimer to the nucleus and the binding of the heterodimer to transcription factors. The AMH-AMHR2 signal plays a crucial role in sex differentiation during development. AMHR2 mediates the regression of the Müllerian Ducts, a crucial step in male sexual development. The absence or loss of function of AMH or AMHR2 can lead to Persistent Müllerian Duct Syndrome (PMDS), a genetic disorder characterized by the persistence of Müllerian Ducts in males. Rat AMHR2 shares a 95% and 82% amino acid sequence identity with mouse and human homologues respectively. During menopausal onset, the ovary expresses certain levels of AMHR2 that maintain the delicate balance of hormonal signaling necessary for normal ovarian function. However, at postmenopausal stage, the ovarian function and level of AMHR2 decreases substantially. Overexpression of AMHR2 (Anti-Müllerian Hormone Receptor type 2) has been observed in a majority of epithelial ovarian carcinomas. The presence of AMHR2 overexpression in ovarian carcinomas has implications for potential targeted therapies. In addition, AMH/AMHR2 signaling have been suggested to negatively regulate lung cancer epithelial-mesenchymal transition (EMT) and chemo-resistance, thus potentially slowing down the progression and spread of lung cancer.
Product DetailsProduct Details
- Source
- Rat AMHR2, amino acid (Ser18-Pro144) (Accession: # Q62893.1), with a linker (GS) and a C-terminal human IgG (Pro100-Lys330), was expressed in CHO cells.
- Molecular Mass
- The 360 amino acid recombinant protein has a predicted molecular mass of approximately 39.43 kD. The DTT-reduced and non-reduced protein migrate at approximately 50 – 62 kD and 75 – 110 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Ser.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.1 EU/per µg cytokine as determined by the LAL method.
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When recombinant human MIS is immobilized at 1 µg/mL, rat AMHR2 binds in a dose-dependent manner with ED50 range of 0.25 – 1.5 µg/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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The mesenchymal cells surrounding the Müellerian duct at embryonic days 14, 15, and 16. Tubular and follicular structures of the fetal gonads
- Function
- Sex differentiation regulation, transcription factor regulation
- Interaction
- Type I receptor including ALK2 and ALK3
- Ligand/Receptor
- MIS/AMH
- Bioactivity
- Rat AMHR2 binds to immobilized recombinant human MIS in a dose-dependent manner.
- Cell Type
- Mesenchymal cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
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- Howard JA, et al. 2022. Front Endocrinol. 13:927824.
- Cate RL. 2022. Front Endocrinol. 13:905324.
- Mullen RD, et al. 2019. Dev Biol. 455:1-9.
- Liu Y, et al. 2022. Genes. 13:159.
- Mazumder S, et al. 2020. Oncotarget. 11:1894-1910.
- Beck TN, et al. 2016. Cell Rep. 16:657-71.
- Mazumder S, et al. 2017. Cancer Prev Res. 10:612-624.
- Kastora SL, et al. 2020. J Assist Reprod Genet. 37:1623-1635.
- Gene ID
- 29530 View all products for this Gene ID
- UniProt
- View information about AMHR2/MIS RII on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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