Spark Blue™ 550 anti-human CD326 (Ep-CAM) (Flexi-Fluor™) Antibody, CD326, 9C4

Pricing & Availability

Clone: 9C4 (See other available formats)
Regulatory Status: RUO
Other Names: Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype: Mouse IgG2b, κ
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285226

50 µg

$394

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Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: DU.4475 breast carcinoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry³, and spatial biology (IBEX)^4,5.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3662530 (BioLegend Cat. No. 285226)

Antigen Details

Structure: Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution: Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.
Function: Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification: Glycosylated.
Cell Type: Embryonic Stem Cells, Epithelial cells
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.
Gene ID: NA
UniProt: View information about CD326 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD326 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD326 (EpCAM)	9C4	FC,ICC,IHC
FITC anti-human CD326 (EpCAM)	9C4	FC
PE anti-human CD326 (EpCAM)	9C4	FC
APC anti-human CD326 (EpCAM)	9C4	FC
Alexa Fluor® 488 anti-human CD326 (EpCAM)	9C4	FC,SB
Alexa Fluor® 647 anti-human CD326 (EpCAM)	9C4	FC,ICC,SB
PerCP/Cyanine5.5 anti-human CD326 (EpCAM)	9C4	FC,SB
Biotin anti-human CD326 (EpCAM)	9C4	FC
Pacific Blue™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 421™ anti-human CD326 (EpCAM)	9C4	FC
PE/Cyanine7 anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 605™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 650™ anti-human CD326 (EpCAM)	9C4	FC
Alexa Fluor® 594 anti-human CD326 (EpCAM)	9C4	IHC-P,ICC,SB
Purified anti-human CD326 (EpCAM) (Maxpar® Ready)	9C4	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD326 (EpCAM)	9C4	FC
APC/Fire™ 750 anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 510™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 785™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 711™ anti-human CD326 (Ep-CAM)	9C4	FC
TotalSeq™-A0123 anti-human CD326 (Ep-CAM)	9C4	PG
APC/Cyanine7 anti-human CD326 (Ep-CAM)	9C4	FC
Alexa Fluor® 700 anti-human CD326 (EpCAM)	9C4	FC
TotalSeq™-C0123 anti-human CD326 (Ep-CAM)	9C4	PG
TotalSeq™-B0123 anti-human CD326 (Ep-CAM)	9C4	PG
PE/Cyanine5 anti-human CD326 (Ep-CAM)	9C4	FC
TotalSeq™-D0123 anti-human CD326 (Ep-CAM)	9C4	PG
Spark UV™ 387 anti-human CD326 (Ep-CAM)	9C4	FC
Spark Red™ 718 anti-human CD326 (Ep-CAM) (Flexi-Fluor™)	9C4	FC
Spark Blue™ 574 anti-human CD326 (Ep-CAM) (Flexi-Fluor™)	9C4	FC
Spark Blue™ 550 anti-human CD326 (Ep-CAM) (Flexi-Fluor™)	9C4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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