- Clone
- SA011F11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Chemokine (C-X3-C motif) receptor 1, Fractalkine receptor, GPR13, CCRL1, CMKBRL1, CMKDR1, V28
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse CX3CR1-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
View more applications data for a different format of this clone in our Scientific Poster Library.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- RRID
-
AB_3662571 (BioLegend Cat. No. 285267)
Antigen Details
- Structure
- G-protein coupled receptor, 7 transmembrane regions, 40 kD.
- Distribution
-
Resident and alternatively activated macrophages (M2), subset of monocytes, dendritic cells (DCs), natural killer cells (NK), subset of memory T cells, mast cells, glial cells, and astrocytes.
- Function
- Involved in cell recruitment during inflammation, participates in cell adhesion and extravasation from blood vessels, coreceptor for HIV1.
- Ligand/Receptor
- CX3CL1 (fractalkine, neurotactin).
- Cell Type
- Astrocytes, Dendritic cells, Macrophages, Mast cells, Microglia, Monocytes, NK cells, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Ponzetta A, et al. 2013. J. Immunol. 191:5684.
2. Jacquelin S, et al. 2013. Blood. 122:674.
3. Garcia JA, et al. 2013. J. Immunol. 191:1063.
4. Lee YS, et al. 2013. Cell. 153:413.
5. Shechter R, et al. 2013. Immunity. 38:555. - Gene ID
- NA
- UniProt
- View information about CX3CR1 on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CX3CR1 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CX3CR1
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Alexa Fluor® 647 anti-mouse CX3CR1
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PE anti-mouse CX3CR1
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APC anti-mouse CX3CR1
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PerCP/Cyanine5.5 anti-mouse CX3CR1
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Brilliant Violet 421™ anti-mouse CX3CR1
-
Brilliant Violet 510™ anti-mouse CX3CR1
-
FITC anti-mouse CX3CR1
-
Alexa Fluor® 488 anti-mouse CX3CR1
-
PE/Dazzle™ 594 anti-mouse CX3CR1
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PE/Cyanine7 anti-mouse CX3CR1
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Biotin anti-mouse CX3CR1
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Brilliant Violet 605™ anti-mouse CX3CR1
-
Brilliant Violet 785™ anti-mouse CX3CR1
-
Brilliant Violet 711™ anti-mouse CX3CR1
-
Brilliant Violet 650™ anti-mouse CX3CR1
-
Alexa Fluor® 700 anti-mouse CX3CR1
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APC/Fire™ 750 anti-mouse CX3CR1
-
Pacific Blue™ anti-mouse CX3CR1
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TotalSeq™-A0563 anti-mouse CX3CR1
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TotalSeq™-C0563 anti-mouse CX3CR1
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TotalSeq™-B0563 anti-mouse CX3CR1
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APC/Cyanine7 anti-mouse CX3CR1 Antibody
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PE/Cyanine5 anti-mouse CX3CR1
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PE/Fire™ 700 anti-mouse CX3CR1
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APC/Fire™ 810 anti-mouse CX3CR1
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PE/Fire™ 640 anti-mouse CX3CR1
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PE/Fire™ 810 anti-mouse CX3CR1
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PerCP/Fire™ 806 anti-mouse CX3CR1
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Brilliant Violet 570™ anti-mouse CX3CR1
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PerCP/Fire™ 780 anti-mouse CX3CR1
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Spark Red™ 718 anti-mouse CX3CR1 (Flexi-Fluor™)
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Spark PLUS UV395™ anti-mouse CX3CR1
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Spark Blue™ 574 anti-mouse CX3CR1 (Flexi-Fluor™)
-
Spark Blue™ 550 anti-mouse CX3CR1 (Flexi-Fluor™)
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