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Spark PLUS B550™ anti-human CD45RO Antibody

Pricing & Availability
Clone
S19021B (See other available formats)
Regulatory Status
RUO
Other Names
PTPRC, CD45R, B220, T200, GP180
Isotype
Mouse IgG1, λ
Ave. Rating
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Product Citations
publications
S19021B_Spark-PLUS-B550_-CD45RO_FC_071824
Human peripheral blood lymphocytes were stained with anti-human CD45RA (clone HI100) Spark Red™ 718 and anti-human CD45RO (clone S19021B) Spark PLUS B550™ (left) or stained with anti-human CD45RA (clone HI100) Spark Red™ 718 only (right).
  • S19021B_Spark-PLUS-B550_-CD45RO_FC_071824
    Human peripheral blood lymphocytes were stained with anti-human CD45RA (clone HI100) Spark Red™ 718 and anti-human CD45RO (clone S19021B) Spark PLUS B550™ (left) or stained with anti-human CD45RA (clone HI100) Spark Red™ 718 only (right).
Compare all formats See Spark PLUS B550™ spectral data
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376811 25 tests $125
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376812 100 tests $310
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Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic human CD45 peptide spanning the junction region between exons 3 and 7.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark PLUS B550™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark PLUS B550™ has a maximum excitation of 516 nm and a maximum emission of 540 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The S19021B antibody can be used in combination with antibodies against CD45RA to discern memory and naïve T cells. Staining profile by S19021B on human peripheral blood lymphocyte is comparable to that by clone UCHL1.

Staining signal by clone S19021B on target cells can be completely blocked by pre-incubation with clone UCHL1; staining of UCHL1 on target cells can be partially blocked by pre-incubation with clone S19021B.

Staining signal by clone S19021B can be significantly increased on the cells after neuraminidase treatment, suggesting that S19021B binding epitope is likely affected by glycosylation.

RRID
AB_3662277 (BioLegend Cat. No. 376811)
AB_3662277 (BioLegend Cat. No. 376812)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution

Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes

Function
Enhances TCR and BCR signaling
Cell Type
B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu Rev Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu Rev Immunol. 12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RO on UniProt.org
Go To Top Version: 1    Revision Date: 07/11/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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