- Clone
- QA17A29 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- EMR1, Ly71
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Murine macrophages
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- RRID
-
AB_3106356 (BioLegend Cat. No. 285102)
Antigen Details
- Structure
- A 160 kD glycoprotein and a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family
- Distribution
-
Expressed on the majority of tissue macrophages
- Function
- Required for induction of CD8+ T cells-mediated peripheral tolerance
- Cell Type
- Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
- Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035.
- Stevceva L, et al. 2001. BMC Clin Pathol. 1:3.
- Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354.
- Poeckel D, et al. 2009. J. Biol Chem. 284:21077.
- Glass AM, et al. 2013. J. Immunol. 190:4830.
- Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570.
- Rankin AL, et al. 2010. J. Immunol. 184:1526.
- Sasi SP, et al. 2014. J Biol Chem. 289:14178.
- Thakus VS, et al. 2014. Toxicol Lett. 230:322.
- Ohnishi A, et al. 2015. PLoS One. 10:117616.
- Gene ID
- 13733 View all products for this Gene ID
- UniProt
- View information about F4/80 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All F4/80 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
PE anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
APC anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
PE/Cyanine7 anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
FITC anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
Spark YG™ 593 anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
APC/Cyanine7 anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
PerCP/Cyanine5.5 anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
Alexa Fluor® 647 anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
PE/Fire™ 640 anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
Spark Red™ 718 anti-mouse F4/80 Recombinant (Flexi-Fluor™) Antibody | QA17A29 | FC |
PE/Fire™ 810 anti-mouse F4/80 Recombinant Antibody | QA17A29 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse F4/80 Recombinant Antibody
-
PE anti-mouse F4/80 Recombinant Antibody
-
APC anti-mouse F4/80 Recombinant Antibody
-
PE/Cyanine7 anti-mouse F4/80 Recombinant Antibody
-
FITC anti-mouse F4/80 Recombinant Antibody
-
Spark YG™ 593 anti-mouse F4/80 Recombinant Antibody
-
APC/Cyanine7 anti-mouse F4/80 Recombinant Antibody
-
PerCP/Cyanine5.5 anti-mouse F4/80 Recombinant Antibody
-
Alexa Fluor® 647 anti-mouse F4/80 Recombinant Antibody
-
PE/Fire™ 640 anti-mouse F4/80 Recombinant Antibody
-
Spark Red™ 718 anti-mouse F4/80 Recombinant (Flexi-Fluor™) Antibody
-
PE/Fire™ 810 anti-mouse F4/80 Recombinant Antibody
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