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Spark Violet™ 423 anti-human CD45 Recombinant Antibody

Pricing & Availability
Clone
QA19A35 (See other available formats)
Regulatory Status
RUO
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A35_SparkViolet423_CD45_Antibody_111821
Human peripheral blood lymphocytes were stained with anti-human CD45 recombinant (clone QA19A35) Spark Violet™ 423 (filled histogram). Open histogram represents unstained cells.
  • QA19A35_SparkViolet423_CD45_Antibody_111821
    Human peripheral blood lymphocytes were stained with anti-human CD45 recombinant (clone QA19A35) Spark Violet™ 423 (filled histogram). Open histogram represents unstained cells.
See Spark Violet™ 423 spectral data
Cat # Size Price Quantity Check Availability Save
311803 25 tests $141
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311804 100 tests $370
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.

Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone QA19A35 antibody is able to completely block binding of clone HI30 on target cells.

RRID
AB_2922545 (BioLegend Cat. No. 311803)
AB_2922545 (BioLegend Cat. No. 311804)

Antigen Details

Structure
Type I transmembrane protein, 180-240 kD
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
Tyrosine phosphatases
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Leukocytes, Mesenchymal Stem Cells
Biology Area
Adaptive Immunity, Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339-69.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85-116.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/18/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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