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Spark Violet™ 423 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Spark Violet™, SAv-Spark Violet™ 423, streptavidin Spark Violet™ 423
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Product Citations
publications
Streptavidin_SparkViolet423_Antibody_FC_041723
Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-Spark Violet™ 423 and anti-human CD19 APC (left) or biotinylated mouse IgG1, κ isotype control, followed with SAV-Spark Violet™ 423 and anti-human CD19 APC (right).
  • Streptavidin_SparkViolet423_Antibody_FC_041723
    Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-Spark Violet™ 423 and anti-human CD19 APC (left) or biotinylated mouse IgG1, κ isotype control, followed with SAV-Spark Violet™ 423 and anti-human CD19 APC (right).
See Spark Violet™ 423 spectral data
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405353 25 µg $124
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405354 100 µg $417
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Description

Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
Streptavidin is conjugated with Spark Violet™ 423 under optimal conditions
Concentration
0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Spark Violet™ 423 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this Streptavidin-Spark Violet™ 423 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark Violet™ 423 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.06 µg per 106 cells in 100 µL staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.

Excitation Laser
Violet Laser (405 nm)

Related FAQs

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Go To Top Version: 1    Revision Date: 04/17/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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