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Spark YG™ 570 anti-NSE Antibody

Pricing & Availability
Clone
NSE-P1 (See other available formats)
Regulatory Status
RUO
Other Names
Gamma-enolase, neural enolase, neuron-specific enolase, 2-phospho-D-glycerate hydrolyase, ENO2, NSE
Isotype
Mouse IgG1, κ
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Product Citations
publications
NSE-P1_SPKYG570_NSE_Antibody_1_032620.png
IHC staining of Spark YG™ 570 anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using sodium citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 50 µm
  • NSE-P1_SPKYG570_NSE_Antibody_1_032620.png
    IHC staining of Spark YG™ 570 anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using sodium citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 50 µm
  • NSE-P1_SPKYG570_NSE_Antibody_2_032620.png
    IHC staining of Spark YG™ 570 anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using sodium citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • NSE-P1_SPKYG570_NSE_Antibody_3_032620.png
    IHC staining of Spark YG™ 570 anti-NSE antibody (clone NSE-P1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using sodium citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Compare all formats See Spark YG™ 570 spectral data
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804911 25 µg $124
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804912 100 µg $311
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Description

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers. Expression of these isozymes can be cell-type and development-specific. This gene encodes gamma enolase (enolase 2) and is expressed in mature neurons and cells of neuronal origin. The alpha/alpha homodimers are expressed in embryo and in most adult tissues. The alpha/beta heterodimers and the beta/beta homodimers are found in striated muscle, and the alpha/gamma heterodimers and the gamma/gamma homodimers are expressed in neurons. Neuron specific enolase is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acids 416-433. It recognizes the sequence LGDEARFAGHNFRNPSVL.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 2 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The reactivity of Alexa Fluor® 647 and Alexa Fluor® 488 anti-NSE antibody was verified in FFPE mouse and rat tissue.

Additional reported applications (for the relevant formats) include: ELISA2 and immunohistochemistry1,3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoo J, et al. 2002. Arch. Pathol. Lab Med. 126(8):979. (IHC-P)
  2. Duncan ME, et al. 1992. J. Immuno. Methods. 151:227 (ELISA)
  3. Chetty R. 2000. Pathology. 32(3):209. (IHC-P)
  4. Murray GI, et al. 1993. J. Clin. Pathol. 46:993. (IHC-P)
RRID
AB_2832852 (BioLegend Cat. No. 804911)
AB_2832852 (BioLegend Cat. No. 804912)

Antigen Details

Structure
Gamma enolase is a 434 amino acid protein with a molecular mass of 47 kD.
Distribution

Tissue distribution: Nervous and musculoskeletal systems.
Cellular distribution: Secreted, cytosol, plasma membrane, cytoskeleton, and nucleus.

Function
Gamma enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Cell Type
Mature Neurons, Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Enzymes and Regulators, Phospho-Proteins
Antigen References
  1. Muoio B, et al. 2017. Int J Biol Markers. 10.5301/ijbm.5000286.
  2. Huang L, et al. 2017. Oncotarget. 8(38): 64358.
  3. Isgro MA, et al. 2015. Adv Exp Med Biol. 867:125.
  4. Zaheer S, et al. 2013. Ann Indian Acad Neurol. 16(4):504.
Gene ID
2026 View all products for this Gene ID
UniProt
View information about NSE on UniProt.org
Go To Top Version: 1    Revision Date: 03/26/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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