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Spark YG™ 581 anti-human CD300e (IREM-2, CMRF35-A5) Antibody

Pricing & Availability
Clone
UP-H2 (See other available formats)
Regulatory Status
RUO
Other Names
CD300e, CMRF35-A5, LMIR6
Isotype
Mouse IgG1, κ
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Product Citations
publications
UP-H2_spark-yg-581_-CD300E_Antibody_101623
Human peripheral blood monocytes were stained with anti-human CD14 Brilliant Violet 421™ and anti-human CD300e (IREM-2, CMRF35-A5) (clone UP-H2) Spark YG™ 581 (left) or mouse IgG1, κ Spark YG™ 581 isotype control (right).
  • UP-H2_spark-yg-581_-CD300E_Antibody_101623
    Human peripheral blood monocytes were stained with anti-human CD14 Brilliant Violet 421™ and anti-human CD300e (IREM-2, CMRF35-A5) (clone UP-H2) Spark YG™ 581 (left) or mouse IgG1, κ Spark YG™ 581 isotype control (right).
Compare all formats See Spark YG™ 581 spectral data
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339707 25 tests $153
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339708 100 tests $364
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Description

IREM-2 (immune receptor expressed by myeloid cells 2), also known as CMRF35-A5, LMIR6, CD300Le or CD300e, is a 34 kD type I single transmembrane glycoprotein. It is a member of the CD300 family and of the Ig-superfamily. IREM-2 is expressed on monocyte and myeloid dendritic cells, but not on granulocytes and lymphoid dendritic cells. IREM-2 is an activating receptor through interaction with DAP12.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
IREM-IgG fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm.

Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunoprecipitation and stimulation of TNF-a production by monocytes

Application References

(PubMed link indicates BioLegend citation)
  1. Aguilar H, et al. 2004. J. Immunol. 173:6703
  2. Gasiorowski RE, et al. 2013. Immunol Lett. 149:93. PubMed
RRID
AB_3083320 (BioLegend Cat. No. 339707)
AB_3083320 (BioLegend Cat. No. 339708)

Antigen Details

Structure
34 kD, type I transmembrane glycoprotein, CD300 family, Ig superfamily
Distribution

Monocytes, macrophages, dendritic cells

Function
Activation of myeloid cells
Cell Type
Dendritic cells, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Aguilar H, et al. 2004. J. Immunol. 173:6703

Gene ID
342510 View all products for this Gene ID
UniProt
View information about CD300e on UniProt.org
Go To Top Version: 1    Revision Date: 10/16/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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