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TMB High Sensitivity Substrate Solution

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Regulatory Status
RUO
Other Names
TMB HS Substrate
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Product Citations
publications
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421501 60 mL $30
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Description

The TMB High Sensitivity (TMB HS) Substrate solution is an one-step working solution for HRP (horse-radish peroxidase) labeled conjugates. TMB HS substrate solution provides high signal and high sensitivity for ELISA. It is recommended for cytokine ELISA reagent sets. The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2N H2SO4 or 1M H3PO4 (resulting in a yellow color), and read at 450 nm.

Product Details
Technical data sheet

Product Details

Storage & Handling
The TMB High Sensitivity Substrate Solution should be stored between 2°C and 8°C. Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.
Application

ELISA
Application References

(PubMed link indicates BioLegend citation)
  1. Zeng S, et al. 2008. Int Immunopharmacol. 10:1707. PubMed
  2. Eisenach PA, et al. 2010. J. Cell Sci. 123:4182. PubMed
  3. Fan YY, et al. 2012. J Lipid Res. 53:1287. PubMed
  4. Querol L, et al. 2014. Neurology. 82:879. PubMed
  5. Kiianitsa K, et al. 2104. Nucleic Acids Res. 42:108. PubMed
  6. Nogales A, et al. 2014. J Virol. 88:10525. PubMed
Product Citations
  1. Querol L, et al. 2014. Neurology. 82:879. PubMed
  2. Fan Y, et al. 2012. J Lipid Res. 53:1287. PubMed
  3. Cho M, et al. 2016. J Immunol. 197: 1065 - 1073. PubMed
  4. Nogales A, et al. 2017. J Virol. 10.1128/JVI.00720-17. PubMed
  5. Zeng S, et al. 2008. Int Immunopharmacol. 10:1707. PubMed
  6. Sheppard S, et al. 2017. Nat Commun. 8:13930. PubMed
  7. Maizels K 2014. Nucleic Acids Res. 42:108. PubMed
  8. Eisenach P, et al. 2010. J Cell Sci. 123:4182. PubMed
  9. Sinha D, et al. 2020. Anal Biochem. 113827:608. PubMed
  10. Nogales A, et al. 2014. J Virol. 88:10525. PubMed
  11. Rother N, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01136. PubMed

Antigen Details

Gene ID
NA

Related FAQs

What is the difference between your two substrate solutions: TMB Substrate Reagent Set (Cat. No. 421101) and TMB High Sensitivity Substrate Solution (Cat. No. 421501)?

TMB Substrate Reagent Set (Cat. No. 421101) contains two components, which need to be mixed immediately prior to use. The TMB High Sensitivity Substrate Solution (Cat. No. 421501) contains everything in one solution with special stabilizers. It is a high kinetic substrate solution which generally gives higher signal. The type of substrate used for an assay can affect the optical density (OD) achievable and the time required to achieve the desired OD.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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