- Clone
- MI15 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- B-B4
- Isotype
- Mouse IgG1, κ
- Barcode Sequence
- ACTCTTTCGTTTACG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
356545 | 10 µg | $369 |
CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells, but not on mature circulating B-lymphocytes. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial cells, and neural cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A mixture of U266 and XG-1 human myeloma cell lines.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.4
Additional reported applications for the relevant formats include: immunofluorescent staining1, Western blotting2, immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections3, and spatial biology (IBEX)5,6. - Additional Product Notes
-
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes. -
Application References
(PubMed link indicates BioLegend citation) -
- Costes V, et al. 1999. Hum. Pathol. 30:1405. (IF)
- Gattei V, et al. 1999. Br. J. Haematol. 104:152. (WB)
- Bologna-Molina R, et al. 2008. Oral Oncol. 44:805. (IHC)
- Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482.
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- RRID
-
AB_2904378 (BioLegend Cat. No. 356545)
Antigen Details
- Structure
- 100-200 kD type I integral transmembrane glycoprotein
- Distribution
-
Plasma cells, pre-B cells, epithelial cells, endothelial cells
- Function
- Adhesion, controls cell morphology, regulates cell growth
- Ligand/Receptor
- FGFb, collagen, fibronectin
- Cell Type
- B cells, Endothelial cells, Epithelial cells, Plasma cells
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. Wiley-Liss A John Wiley & Sons Inc, Publication. - Gene ID
- 6382 View all products for this Gene ID
- UniProt
- View information about CD138 on UniProt.org
Related FAQs
Other Formats
View All CD138 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... Confocal image of human lymph node sample acquired using the... Confocal image of human liver sample acquired using the IBEX... Confocal image of human jejunum sample acquired using the IB... -
Purified anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with purified CD138... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
APC anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
FITC anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (Syndeca... -
Alexa Fluor® 700 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
PE/Cyanine7 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Brilliant Violet 421™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Brilliant Violet 510™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Brilliant Violet 605™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Brilliant Violet 711™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Alexa Fluor® 647 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... Confocal image of human skin sample acquired using the IBEX ... Confocal image of human metastatic lymph node sample acquire... -
Alexa Fluor® 594 anti-human CD138 (Syndecan-1)
U266B1 cells were fixed with 2% paraformaldehyde (PFA) for t... -
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
APC/Cyanine7 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Pacific Blue™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with Pacific Blue™ ... -
TotalSeq™-A0055 anti-human CD138 (Syndecan-1)
-
Brilliant Violet 785™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (clone M... -
Biotin anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (Syndeca... -
TotalSeq™-C0055 anti-human CD138 (Syndecan-1)
-
APC/Fire™ 750 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with CD138 (Syndeca... -
TotalSeq™-B0055 anti-human CD138 (Syndecan-1)
-
PE/Cyanine5 anti-human CD138 (Syndecan-1)
U266 (human myeloma cell line) cells were stained with anti-... -
TotalSeq™-D0055 anti-human CD138 (Syndecan-1)
-
PE/Fire™ 640 anti-human CD138 (Syndecan-1)
U266 cells were stained with anti-human CD138 (Syndecan-1) (... -
Spark Violet™ 500 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with anti-human CD1... -
FITC anti-human CD138
Typical results from human myeloma cell line U266 stained ei... -
PE/Fire™ 700 anti-human CD138 (Syndecan-1)
U266 cells were stained with anti-human CD138 (Syndecan-1) (... -
Spark Violet™ 423 anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with anti-human CD1... -
Spark Red™ 718 anti-human CD138 (Syndecan-1) (Flexi-Fluor™)
-
Brilliant Violet 650™ anti-human CD138 (Syndecan-1)
Human myeloma cell line U266 was stained with anti-human CD1...
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