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Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Tris-EDTA H.I.E.R. pH 9.0, 10X, Tris-EDTA AR Buffer
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publications
Tris-EDTA-pH-90-Antigen-Retrieval-Buffer_020124
IHC staining on formalin-fixed paraffin-embedded human brain. Tissues treated with (panel A) or without (panel B) the heating step for Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) were incubated with 2 µg/mL of Purified anti-GFAP (clone 2E1.E9) (Cat. No. 644702) primary antibody. Then, secondary staining was performed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (magenta) for 1 hour at room temperature, and co-stained with 10 µg/mL of Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3) (clone TUJ1) (Cat. No. 801203) (green). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured with a 10X objective. Scale bar: 50 µm
  • Tris-EDTA-pH-90-Antigen-Retrieval-Buffer_020124
    IHC staining on formalin-fixed paraffin-embedded human brain. Tissues treated with (panel A) or without (panel B) the heating step for Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) were incubated with 2 µg/mL of Purified anti-GFAP (clone 2E1.E9) (Cat. No. 644702) primary antibody. Then, secondary staining was performed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (magenta) for 1 hour at room temperature, and co-stained with 10 µg/mL of Alexa Fluor® 488 anti-Tubulin β 3 (TUBB3) (clone TUJ1) (Cat. No. 801203) (green). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). Images were captured with a 10X objective. Scale bar: 50 µm
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422703 100 mL $70
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422704 500 mL $150
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Description

Tris-EDTA pH 9.0 antigen retrieval buffer, 10X is a universal antigen unmasking reagent to be used with heat induced epitope retrieval (H.I.E.R).

Product Details
Technical data sheet

Product Details

Storage & Handling
Store at room temperature.
Application

IHC-P - Quality tested
Recommended Usage

Dilute to 1X with DI water before usage.

The optimal antigen unmasking condition is a function of temperature (90 to 100°C) and incubation time (5 - 30 minutes) and should be established by the end user.

Application Notes

For acidic antigen retrieval buffer (pH 6.0) refer to BioLegend’s Citrate Buffer,10X (Cat. No. 420902) and Sodium Citrate H.I.E.R., 1x (Cat. No. 928502)

For neutral antigen retrieval buffer (pH 7.6) refer to BioLegend’s Tris-Buffered Saline 20X (1.0 M) (Cat. No. 925601) and Tris-Buffered Saline 20X (1.0 M) with Tween 20 (Cat. No. 925501)

For other pH value antigen retrieval buffers, refer to Retrieve-All Antigen Unmasking system:

Retrieve-All 1 (Universal pH 8) (Cat. No. 928101)
Retrieve-All 2 (Basic pH 10) (Cat. No. 928401)
Retrieve-All 3 (Acidic pH 4.8) (Cat. No. 927601)

Antigen Details

Gene ID
NA

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Go To Top Version: 1    Revision Date: 02/01/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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