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Ultra-LEAF™ Purified anti-CXCL12 (SDF-1β) Antibody

Pricing & Availability
Clone
W15149B (See other available formats)
Regulatory Status
RUO
Other Names
Bone morphogenetic protein 14 (BMP14), cartilage-derived morphogenetic protein 1 (CDMP1), BDA1C, Lipopolysaccharide Pre-B-cell growth-stimulating factor (PBSF), stromal cell derived factor 1 isoform beta precursor (SDF-1β)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W15149B_ULEAF_CXCL12_SDF-1beta_Antibody_a_062121.png
Recombinant mouse CXCL12 (SDF-1β) (Cat. No. 589806) (black circles) induces the chemotaxis of mouse Baf3-hCXCR4 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-CXCL12 (SDF-1β) antibody (clone W15149B) (purple squares) neutralizes the chemotaxis of recombinant mouse CXCL12 (SDF-1β) on mouse Baf3-hCXCR4 cells in a dose-dependent manner. ND50 range: 0.05 - 0.3 µg/mL.
  • W15149B_ULEAF_CXCL12_SDF-1beta_Antibody_a_062121.png
    Recombinant mouse CXCL12 (SDF-1β) (Cat. No. 589806) (black circles) induces the chemotaxis of mouse Baf3-hCXCR4 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-CXCL12 (SDF-1β) antibody (clone W15149B) (purple squares) neutralizes the chemotaxis of recombinant mouse CXCL12 (SDF-1β) on mouse Baf3-hCXCR4 cells in a dose-dependent manner. ND50 range: 0.05 - 0.3 µg/mL.
  • W15149B_ULEAF_CXCL12_SDF-1beta_Antibody_b_062121.png
    Recombinant mouse CXCL12 (SDF-1α) (Cat. No. 578706) (black circles) induces the chemotaxis of mouse Baf3-hCXCR4 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-CXCL12 (SDF-1β) antibody (clone W15149B) (purple squares) neutralizes the chemotaxis of recombinant mouse CXCL12 (SDF-1α) on mouse Baf3-hCXCR4 cells in a dose-dependent manner. ND50 range: 0.05 - 0.3 µg/mL.
  • W15149B_ULEAF_CXCL12_SDF-1beta_Antibody_c_062121.png
    Recombinant human CXCL12 (SDF-1α) (Cat. No. 581206) (black circles) induces the chemotaxis of mouse Baf3-hCXCR4 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-CXCL12 (SDF-1β) antibody (clone W15149B) (purple squares) neutralizes the chemotaxis of recombinant human CXCL12 (SDF-1α) on mouse Baf3-hCXCR4 cells in a dose-dependent manner. ND50 range: 0.02 - 0.12 µg/mL.
  • W15149B_ULEAF_CXCL12_SDF-1beta_Antibody_d_062121.png
    Recombinant human CXCL12 (SDF-1β) (Black Circles, Cat. No. 587706) induces the chemotaxis of mouse Baf3-hCXCR4 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-CXCL12 (SDF-1β) antibody (clone W15149B) (purple squares) neutralizes the chemotaxis of recombinant human CXCL12 (SDF-1β) on mouse Baf3-hCXCR4 cells in a dose-dependent manner. ND50 range: 0.8 - 4 µg/mL.
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939903 100 µg $270
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939904 1 mg $715
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Description

CXCL12 is commonly known as SDF-1, with two variants, SDF-1α (89 amino acids) and SDF-1β (93 amino acids) derived from alternative splicing. They have identical amino acid sequences, differing only by the addition of four amino acids at the carboxy-terminus of the β form. CXCL12β is constitutively expressed by stromal cells. CXCL12α and β are ubiquitously expressed, and it has been suggested that their activity is regulated through differential proteolytic processing. Proteases present in serum process CXCL12α initially at the carboxy-terminus (through carboxypeptidase N) and subsequently at the amino-terminus (through CD26); while CXCL12β is only processed at the amino-terminus. These proteolytic processes affect the chemokine's ability to bind to heparin and cells as well as its ability to stimulate proliferation and chemotaxis. CXCL12α is inactivated by other enzymes such as metalloproteases, serine proteases, and leukocyte elastase. CXCL12/CXCR4 pair plays a key role in maintaining proliferation and survival of bone marrow stem cell subpopulations under stress conditions. Also, CXCL12 is highly expressed in bone marrow stromal stem cells and regulates growth and survival. CXCL12 promotes survival by inducing anti-apoptotic proteins, decreasing apoptotic proteins, and increasing transcription of cell survival genes. Data suggests that CXCL12β mediates cells' survival by enhancing autophagy in bone marrow mesenchymal stem cells. CXCL12 also plays important role in neurogenesis, neuroinflammation in CNS.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CXCL12 (SDF-1β)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CXCL12 (SDF-1β) on mouse Baf3-hCXCR4 transfectant chemoattractant cells. ND50 range: 0.05 - 0.3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant mouse CXCL12 (SDF-1α) on mouse Baf3-hCXCR4 transfectant chemoattractant cells. ND50 range: 0.05 - 0.3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CXCL12 (SDF-1α) on mouse Baf3-hCXCR4 transfectant chemoattractant cells. ND50 range: 0.02 - 0.12 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CXCL12 (SDF-1β) on mouse Baf3-hCXCR4 transfectant chemoattractant cells. ND50 range: 0.8 - 4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894525 (BioLegend Cat. No. 939903)
AB_2894525 (BioLegend Cat. No. 939904)

Antigen Details

Structure
Chemokine
Distribution

Expressed by bone marrow stromal cells, pericytes, fibroblasts, endothelial cells, epithelial cells, dendritic cells, astrocytes, hematopoietic stem and progenitors, leukocytes, lymphocytes, macrophages, megakaryocytes, microglia, monocytes, neuron stem cells, neurons

Function
CXCL12 is a constitutive chemokine involved in lung, brain, joint, and intestine inflammation. CXCL12 is chemotactic for T, B, and cancer cells. CXCL12/CXCR4 pair plays a role in mobilization of CD34+ progenitors to peripheral blood. CXCL12 is important in morphogenesis, angiogenesis, cancer metastasis, neurogenesis and neuroinflammation.
Interaction
Endothelial cells, epithelial cells, T and B cells, monocytes
Ligand/Receptor
CXCR4
Cell Type
Astrocytes, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Leukocytes, Lymphocytes, Macrophages, Megakaryocytes, Microglia, Monocytes, Neural Stem Cells, Neurons
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Death, Immuno-Oncology, Immunology, Innate Immunity, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Stem Cells
Molecular Family
Adhesion Molecules, Cytokines/Chemokines, GPCR
Antigen References

1. Nagasawa T, et al. 1994. Proc Natl Acad Sci USA. 91:2305.
2. Muller A, et al. 2001. Nature. 410:50.
3. Valenzuela-Fernandez A, et al. 2002. J Biol Chem. 277:15677.
4. De La Luz Sierra M, et al. 2004. Blood. 103:2452.
5. Kortesidis A, et al. 2005. Blood. 105:3793.
6. Davis DA, et al. 2005. Blood. 105:4561.
7. Zlotnik A. 2008. J Pathol. 215:211.
8. Herberg S, et al. 2013. PLoS One. 8:e58207.

Gene ID
6387 View all products for this Gene ID
UniProt
View information about CXCL12 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 09/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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