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Ultra-LEAF™ Purified anti-human CCL11 (Eotaxin) Antibody

Pricing & Availability
Clone
A16056E (See other available formats)
Regulatory Status
RUO
Other Names
Eotaxin, eotaxin-1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A16056E_ULEAF_CCL11_Antibody_040722
Recombinant human CCL11 (Eotaxin) (Cat. No. 583002) (black circles) induces the chemotaxis of mouse Baf3-mCCR3 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CCL11 (Eotaxin) (A16056E) (purple squares) neutralizes the chemotaxis of recombinant human CCL11 (Eotaxin) on mouse Baf3-mCCR3 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 400264) (gray triangles) does not have the effect. ND50 range: 0.1 - 0.5 µg/mL.
  • A16056E_ULEAF_CCL11_Antibody_040722
    Recombinant human CCL11 (Eotaxin) (Cat. No. 583002) (black circles) induces the chemotaxis of mouse Baf3-mCCR3 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CCL11 (Eotaxin) (A16056E) (purple squares) neutralizes the chemotaxis of recombinant human CCL11 (Eotaxin) on mouse Baf3-mCCR3 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 400264) (gray triangles) does not have the effect. ND50 range: 0.1 - 0.5 µg/mL.
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601753 100 µg $358
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601754 1 mg $1205
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Description

CCL11 is a member of the CC chemokine family and was originally identified as a chemoattractant for eosinophils. In response to allergens, CCL11, which participates in a variety of allergic diseases, promotes accumulation of eosinophils. In addition, CCL11 facilitates proliferation and metastasis of tumor cells expressing the CCL11 receptor, CCR3. CCL11 has been shown to induce angiogenic response by CCR3-positive microvascular endothelial cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL11 (Eotaxin)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant human CCL11 (Eotaxin) (Cat. No. 583002) on mouse Baf3-mCCR3 transfectant chemoattractant cells. ND50 range: 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922630 (BioLegend Cat. No. 601753)
AB_2922630 (BioLegend Cat. No. 601754)

Antigen Details

Structure
Chemokine
Distribution

Epithelial cells, smooth muscle cells, dendritic cells, and fibroblasts

Function
CCL11 selectively recruits eosinophils through binding to the chemokine receptor CCR3 during allergic response. CCL11 production is upregulated in response to several proimflammatory cytokines, such as IL-4, IL-13, TNF-α, and IFN-α, produced by mast cells and Th2 lymphocytes. CCL11 has been reported to stimulate eosinophil release from bone marrow, resulting in an elevated level of circulating eosinophils.
Interaction
Eosinophils
Ligand/Receptor
CCR3
Cell Type
Dendritic cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Conroy DM, et al. 2001. Respir Res. 2:150-6.
  2. Levina V, et al. 2009. Clin Cancer Res. 15:2647-56.
  3. Salcedo R, et al. 2001. J Immunol. 166:7571-8.
  4. Kaehler J, et al. 2006. J Investig Med. 54:446-54.
  5. Hadjicharalambous C, et al. 2004. J Allergy Clin Immunol. 113:657-62.
  6. Crump MP, et al. 1998. J Biol Chem. 273:22471-9.
Gene ID
6356 View all products for this Gene ID
UniProt
View information about CCL11 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 09/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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