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Ultra-LEAF™ Purified anti-human CD357 (GITR) Antibody

Pricing & Availability
Clone
W21071E (See other available formats)
Regulatory Status
RUO
Other Names
Glucocorticoid-induced TNFR-related protein (GITR), CD357, AITR, Energen
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W21071E_ULEAF_CD357_Block_092823
Recombinant Human TNFSF18 (GITRL) (Cat. No. 559204) (black circles) binds to immobilized Recombinant Human GITR (TNFRSF18) Fc Chimera (Cat. No. 764302). Ultra-LEAF™ purified anti-human CD357 (GITR) (clone W21071E) (purple squares) inhibits this binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range: 0.3 - 2 µg/mL
  • W21071E_ULEAF_CD357_Block_092823
    Recombinant Human TNFSF18 (GITRL) (Cat. No. 559204) (black circles) binds to immobilized Recombinant Human GITR (TNFRSF18) Fc Chimera (Cat. No. 764302). Ultra-LEAF™ purified anti-human CD357 (GITR) (clone W21071E) (purple squares) inhibits this binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range: 0.3 - 2 µg/mL
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624753 100 µg $415
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624754 1 mg $1195
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Description

NFRSF18 is a type I transmembrane protein that was initially identified from dexamethasone-treated murine T cell hybridoma cells, and was designated GITR (for glucocorticoid-induced TNFR family-related gene). The human TNFRSF18 has 56% amino acid sequence identity to the mouse receptor. The human and mouse receptors contain three cysteine rich domains (CRD) in their extracellular region. TNFRSF18 expression is up-regulated in human peripheral mononuclear cells after stimulation with anti-CD3/CD28 monoclonal antibodies. TNFRSF18 interacts with TRAF1 (TNF receptor-associated factor 1), TRAF2, and TRAF3, and induces nuclear factor NFkB activation via TRAF2. TNFRSF18 is a key player in the differentiation of thymic Tregs (tTregs), and expansion of both tTregs and peripheral Tregs (pTregs). Human regulatory T cells (Tregs) constitutively expresses TNFRSF18. TNFRSF18 and its ligand are involved in the pathogenesis of autoimmune disease.  Soluble forms for TNFRSF18 and TNFSF18 were detected in the serum of patients with primary Sjögren's syndrome, and those levels correlated with disease severity. Other autoimmune and allergic diseases have been associated to TNFRSF18 /TNFSF18 interaction as demonstrated in disease models of gastritis, thyroiditis, experimental autoimmune encephalomyelitis, and allergic airway inflammation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD357 (GITR)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.15 µg/mL human TNFSF18 (GITRL) to 2 µg/mL immobilized recombinant human GITR (TNFRSF18) Fc Chimera. ND50 range: 0.3-2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3083414 (BioLegend Cat. No. 624753)
AB_3083414 (BioLegend Cat. No. 624754)

Antigen Details

Structure
Homodimer
Distribution

Immature and mature dendritic cells, B cells, macrophages, keratinocytes, endothelial cells, and retinal epithelial cells

Function
Differentiation of thymic Tregs (tTregs), and expansion of both tTregs and peripheral Tregs (pTregs). TNFRSF18 is stimulated in anti-CD3/CD28 activated T cells
Interaction
Macrophages, B cells, dendritic cells (DCs), and endothelial cells
Ligand/Receptor
TNFSF18 (AITRL, GITRL, TL6)
Cell Type
Lymphocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Nocentini G, et al. 1997. Proc. Natl. Acad. Sci. USA. 94:6216-21.
  2. Gurney AL, et al. 1999. Curr Biol. 9:215-8.
  3. Shimizu J, et al. 2002. Nat Immunol. 3:135-42.
  4. Tone M, et al. 2003. Proc Natl Acad Sci USA. 100:15059.
  5. Gan X, et al. 2013. Clin Dev Immunol. 2013:340751.
  6. Nocentini G, et al. 2012. Br J Pharmacol. 165:2089-99.
  7. Ronchetti S, et al. 2015. J Immunol Res. 2015:171520.
Gene ID
8784 View all products for this Gene ID
UniProt
View information about CD357 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/28/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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