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Ultra-LEAF™ Purified anti-human GDF-15 Antibody

Pricing & Availability
Clone
A22095G (See other available formats)
Regulatory Status
RUO
Other Names
MIC-1, NAG-1, pTGFB, PDF, PLAB
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A22095G_ULEAF_GDF-15_Block_112624
Recombinant human GDF-15 (Cat. No. 799204) (black circles) binds to immobilized recombinant GDNF receptor-alpha-like. Ultra-LEAF™ Purified anti-human GDF-15 (A22095G) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ Isotype Control (Cat. No. 400264) (gray triangles) does not have the effect. This antibody blocks the binding of 1 ng/mL human GDF-15 to 0.1 µg/mL immobilized recombinant GDNF receptor-alpha-like. ND50 range: 0.14 - 0.9 µg/mL
  • A22095G_ULEAF_GDF-15_Block_112624
    Recombinant human GDF-15 (Cat. No. 799204) (black circles) binds to immobilized recombinant GDNF receptor-alpha-like. Ultra-LEAF™ Purified anti-human GDF-15 (A22095G) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ Isotype Control (Cat. No. 400264) (gray triangles) does not have the effect. This antibody blocks the binding of 1 ng/mL human GDF-15 to 0.1 µg/mL immobilized recombinant GDNF receptor-alpha-like. ND50 range: 0.14 - 0.9 µg/mL
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695453 100 µg $330
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695454 1 mg $805
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Description

GDF-15 is a member of the TGF-β superfamily. GDF-15 is expressed as a 308 amino acid precursor that includes a signal peptide, a pro-domain, and a 112 residue mature protein. The secreted pro-domain binds to the extracellular matrix, where it can be activated by multiple enzymes such as furin (PCSK3), PCSK5, PCSK6, MMP-26, and MT1-MMP. GDF-15 is mainly expressed in placenta and prostate. Its expression is low in other tissues such as kidney, bladder, stomach, pancreas, liver, and gall bladder. GDF-15 is highly expressed in cancer cells with high levels detected in serum of cancer patients. GDF-15 has an important role in metabolic syndrome. GDF-15 transgenic mice present a lean phenotype. Furthermore, GDF-15 affects the macrophages in adipose tissue.  Lean adipose tissue is characterized by the presence of anti-inflammatory M2-like macrophages. In obese individuals, adipose tissue show presence of CD8 effector cells and recruitment of inflammatory M1 macrophages. The increase of M1 macrophages over M2 results in metabolic changes, increase of TNFα, induction of glucose intolerance, and insulin insensitivity. GDF-15 binds to the GDNF receptor family member alpha like (GFRAL) which interacts with the co-receptor RET.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human GDF-15
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 1 ng/mL human GDF-15 (Cat. No. 799204) to 0.1 µg/mL immobilized recombinant GDNF receptor-alpha-like. ND50 range:  0.14 - 0.9 µg/mL.

RRID
AB_3683502 (BioLegend Cat. No. 695453)
AB_3683502 (BioLegend Cat. No. 695454)

Antigen Details

Structure
Homodimer
Distribution

Macrophages, adipocytes, cardiomyocytes, endothelial, and vascular smooth muscle cells. Expressed mainly in placenta and prostate.

Function
Regulates metabolism, appetite, cell survival, and immune tolerance. Regulated by androgens and calcitriol in prostate. Induced by inflammatory cytokines: IL-1b, TNFa, IL-2, and G-CSF.
Interaction
Neurons, gastric cancer cells, normal gastric mucosal cells, adipose tissue
Ligand/Receptor
GFRAL
Cell Type
Epithelial cells, Macrophages, Neurons
Biology Area
Cancer Biomarkers, Immuno-Oncology
Molecular Family
Growth Factors
Antigen References
  1. Bootcov MR, et al. 1997. Proc Natl Acad Sci USA. 94:11514.
  2. Emmerson PJ, et al. 2017. Nat Med. 23:1215-1219.
  3. Li J, et al. 2018. Mol Cell Biol. 38:e00099-18.
  4. Li S, et al. 2014. J Clin Endocrinol Metab. 99:E2277.
  5. Nishimura S, et al. 2009. Nat Med. 15:914-20.
  6. Lackey DE, et al. 2016. Nat Rev Endocrinol. 12:15-28.
  7. Buchholz K, et al. 2021. J Cancer. 12:4698-4709.
Gene ID
9518 View all products for this Gene ID
UniProt
View information about GDF-15 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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