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Ultra-LEAF™ Purified anti-human Glypican-4 Antibody

Pricing & Availability
Clone
A21050B (See other available formats)
Regulatory Status
RUO
Other Names
GPC4
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
a. A21050B_ULEAF_Glypican-4_ICC_022823
The human embryonal carcinoma cell line NTERA-2 was grown on 96-well plates with a coverslip bottom and were fixed with Fixation Buffer (Cat. No. 420801) for 30 minutes. Cells were washed twice with PBS and stained with 5 µg/mL Ultra-LEAF™ purified anti-human Glypican-4 (clone A21050B) followed by Alexa Fluor® 488 anti-mouse IgG (green). Filamentous Actin was stained with Flash Phalloidin Red 594 (Cat. No. 424203). Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Cells were imaged using a 20x objective.
  • a. A21050B_ULEAF_Glypican-4_ICC_022823
    The human embryonal carcinoma cell line NTERA-2 was grown on 96-well plates with a coverslip bottom and were fixed with Fixation Buffer (Cat. No. 420801) for 30 minutes. Cells were washed twice with PBS and stained with 5 µg/mL Ultra-LEAF™ purified anti-human Glypican-4 (clone A21050B) followed by Alexa Fluor® 488 anti-mouse IgG (green). Filamentous Actin was stained with Flash Phalloidin Red 594 (Cat. No. 424203). Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Cells were imaged using a 20x objective.
  • b. A21050B_ULEAF_Glypican-4_Block_022823
    The human embryonal carcinoma cell line NTERA-2 was grown on 96-well plates with coverslip bottom. Cells were treated with 5 µg/mL of Ultra-LEAF™ purified anti-human Glypican-4 (clone A21050B) or mouse IgG2b, κ isotype control or left untreated (Wnt3a-only control). After 15 minutes, cells were stimulated with 200 ng/mL recombinant human Wnt3a for 3h or left unstimulated. Cells were fixed using Transcription Factor Fix working solution for 30 minutes and washed twice with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Cells were then stained with purified anti-β Catenin (CTNNB1) (Cat. No. 844601) followed by Alexa Fluor® 594 anti-mouse IgG. Fluorescence intensity in the cytoplasm and the nucleus was quantified using ImageJ, and the nuclear/cytoplasmic fluorescence ratio calculated.
  • c. A21050B_ULEAF_Glypican-4_FC_022823
    The human embryonal carcinoma cell line NTERA-2 was stained with Ultra-LEAF™ purified anti-human Glypican-4 (clone A21050B) (filled histogram) or mouse IgG2b, κ isotype control (open histogram) followed by anti-mouse IgG PE.
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386403 100 µg $360
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386404 1 mg $1205
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Description

Glypican-4 is a cell surface heparan sulfate proteoglycan that contains a core protein anchored to the cytoplasmic membrane via a GPI anchor. Glypicans function as coreceptors for a variety of growth factors including Wnt, bone morphogenetic proteins (BMPs), fibroblast growth factors, and Hedgehog. In particular, Glypican-4 enhances the Wnt3a dependent β-catenin pathway and the Wnt5a-dependent non-canonical Wnt signaling. Glypican-4 plays a role in the control of cell division and growth regulation and is upregulated in pancreatic cancer and colorectal cancer where it is involved in promoting chemoresistance. Glypican-4 also regulates glutamatergic synapse formation and function in the developing brain.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
AA 23-529
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ICC - Quality tested
FC, Block - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For blocking Glypican-4 mediated Wnt signaling in vitro, a concentration range of 0.1 – 5.0 μg/mL is suggested.

 

Application Notes

This antibody blocks Glypican-4 mediated Wnt3a signaling in vitro at concentrations from 0.1 - 5.0 µg/mL.

RRID
AB_3083388 (BioLegend Cat. No. 386403)
AB_3083388 (BioLegend Cat. No. 386404)

Antigen Details

Structure
Cell surface heparan sulfate proteoglycan
Distribution

Cell membrane and nucleoplasm

Function
Enhances Wnt signaling by acting as R-spondin co-receptor
Interaction
PICK1, Insulin Receptor, Wnt3a, FGF2
Ligand/Receptor
Wnt3a
Cell Type
Neurons
Biology Area
Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Signal Transduction
Molecular Family
APP/Aβ Degradation
Antigen References
  1. Sakane H, et al. 2012. J Cell Sci. 125:449-60.
  2. Hagihara K, et al. 2000. Developmental Dynamics. 219:353-67.
  3. Watanabe K, et al. 1995. J Cell Biol. 130:1207-18.
  4. Ussar, S. et al. 2012. Diabetes. 61:2289-98. 
Gene ID
2239 View all products for this Gene ID
UniProt
View information about Glypican-4 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/02/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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