- Clone
- L243 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Major Histocompatibility Class II, MHC class II
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Dog, Common Marmoset, Squirrel Monkey, Cotton-topped Tamarin
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
CyTOF® - Verified
IP, WB, Block, IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19
Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669). -
Application References
(PubMed link indicates BioLegend citation) -
- Brodsky F. 1984. Immunogenetics 19:179.
- Robbins P, et al. 1987. Human Immunol. 18:301.
- Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
- Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
- Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
- Zipf T, et al. 1981. Cancer Res. 41:4786.
- Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
- Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
- Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
- Wang RF, et al. 1999. Science 284:1351. (Block)
- Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
- Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
- Stein R, et al. 2011. Leuk. Lymphoma 52:273.
- Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
- Moro M, et al. 2005. BMC Immunol. 6:24.
- Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2561493 (BioLegend Cat. No. 307665)
AB_2561493 (BioLegend Cat. No. 307648)
AB_2561493 (BioLegend Cat. No. 307666)
AB_2561493 (BioLegend Cat. No. 307667)
AB_2561493 (BioLegend Cat. No. 307668)
AB_2561493 (BioLegend Cat. No. 307669)
Antigen Details
- Structure
- Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
- Distribution
-
B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
- Function
- Peptide presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364. - Gene ID
- 3122 View all products for this Gene ID 3123 View all products for this Gene ID
- UniProt
- View information about HLA-DR on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All HLA-DR Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 APC -
FITC anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 FITC -
PE anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE -
PE/Cyanine5 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE/Cyan... -
Purified anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 AP... -
Biotin anti-human HLA-DR
Human peripheral blood lymphocytes stained with biotinylated... -
PE/Cyanine7 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE/Cyan... -
APC/Cyanine7 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
Alexa Fluor® 488 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa F... Confocal image of human lymph node sample acquired using the... Confocal image of human lymph node sample acquired using the... Confocal image of human liver sample acquired using the IBEX... -
Alexa Fluor® 647 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa F... -
Pacific Blue™ anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Pacific... -
Alexa Fluor® 700 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa F... -
PerCP anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PerCP -
PerCP/Cyanine5.5 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 605™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 421™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 570™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 711™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 785™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 510™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Ultra-LEAF™ Purified anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 650™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
Purified anti-human HLA-DR (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 174Yb-an... -
PE/Dazzle™ 594 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
FITC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 PE... -
Pacific Blue™ anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0159 anti-human HLA-DR
-
TotalSeq™-B0159 anti-human HLA-DR
-
TotalSeq™-C0159 anti-human HLA-DR
-
Brilliant Violet 750™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
APC/Fire™ 750 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
PerCP/Cyanine5.5 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 810 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE/Fire™ 640 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 538 anti-human HLA-DR Antibody
Human peripheral blood lymphocytes were stained with CD19 AP... -
KIRAVIA Blue 520™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0159 anti-human HLA-DR
-
PE/Fire™ 810 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE/Dazzle™ 594 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 423 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Cyanine7 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP Pacific Blue™ anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP APC/Fire™ 750 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes and ... -
Spark Violet™ 500 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PerCP/Cyanine5.5 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP PE anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
Spark UV™ 387 anti-human HLA-DR
Human peripheral blood lymphocytes stained with anti-human C... -
Spark Blue™ 515 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark NIR™ 685 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Blue™ 550 anti-human HLA-DR (Flexi-Fluor™)
-
Spark Red™ 718 anti-human HLA-DR (Flexi-Fluor™)
-
PE/Fire™ 744 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS UV395™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Violet™ 423 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai...
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