- Clone
- JES10-5A2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-13, NC30
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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501917 | 100 µg | $204 | ||||
501918 | 1 mg | $655 |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The JES10-5A2 antibody reacts with human interleukin-13 (IL-13). The JES10-5A2 antibody can neutralize the bioactivity of natural or recombinant IL-13.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- COS-expressed, recombinant human IL-13
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
ELISA Capture - Quality tested
ELISPOT Capture, Neut, ICFC, IHC, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 1.0 - 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human IL-13 recombinant protein ranging from 2000 to 8 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA or ELISPOT Capture1,2: The Ultra-LEAF™ Purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (Cat. No. 502001) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture.
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Application References
(PubMed link indicates BioLegend citation) -
- McKenzie, A., et al. 1994. Curr. Prot. Immunol.. John Wiley & Sons. New York. Section 6.18.
- Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
- Schaerli, P., et al. 2000. J. Exp. Med. 192:1553.
- Andersson, J., et al. 1994. Immunology 83:16.
- Skansen-Saphir, U., et al. 1994. Eur. J. Immunol. 24:916.
- Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
- Gelfand, E. W., et al. 2006. J. Aller. and Clinic. Immunol. 117:577.
- Knorr, C., et al. 1999. Am. J. Pathol. 155:2019.
- RRID
-
AB_2820084 (BioLegend Cat. No. 501917)
AB_2820084 (BioLegend Cat. No. 501918)
Antigen Details
- Structure
- Cytokine; 17 kD (Mammalian)
- Bioactivity
- Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
- Cell Sources
- Activated T cells, mast cells, NK cells
- Cell Targets
- B cells, monocytes, macrophages, basophils
- Receptors
- Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497.
3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797.
4. Ochensberger, B., et al. 1996. Blood 88:3028. - Regulation
- IL-13Rα2 possible antagonist
- Gene ID
- 3596 View all products for this Gene ID
- UniProt
- View information about IL-13 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All IL-13 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-human IL-13 | JES10-5A2 | ICFC |
LEAF™ Purified anti-human IL-13 | JES10-5A2 | ELISA Capture, ELISPOT Capture, ICFC, IHC, Neut, WB |
PE anti-human IL-13 | JES10-5A2 | ICFC |
Purified anti-human IL-13 | JES10-5A2 | ELISA Capture,IHC,WB |
PerCP/Cyanine5.5 anti-human IL-13 | JES10-5A2 | ICFC |
PE/Cyanine7 anti-human IL-13 | JES10-5A2 | ICFC |
Brilliant Violet 421™ anti-human IL-13 | JES10-5A2 | ICFC |
Ultra-LEAF™ Purified anti-human IL-13 | JES10-5A2 | ELISA Capture,ELISPOT Capture,ICFC,IHC,Neut,WB |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human IL-13
Enriched human CD4+ T cells were stimulated with PMA+ionomyc... -
LEAF™ Purified anti-human IL-13
-
PE anti-human IL-13
PMA+ionomycin-stimulated T lymphocytes intracellularly stain... -
Purified anti-human IL-13
-
PerCP/Cyanine5.5 anti-human IL-13
PMA-restimulated Th2 polarized human peripheral blood lympho... -
PE/Cyanine7 anti-human IL-13
TH2 polarized human peripheral blood lymphocytes were surfac... -
Brilliant Violet 421™ anti-human IL-13
TH2 polarized human peripheral blood lymphocytes were surfac... -
Ultra-LEAF™ Purified anti-human IL-13
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