Ultra-LEAF™ Purified anti-human IL-1α Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human IL-1α

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
ELISPOT Capture, ICFC, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1.0 - 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 200 to 1.56 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture¹: The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1a (Cat. No. 570009) as the standard. The Ultra-LEAF™ purified antibody (Cat. No. 500108) is suggested for ELISPOT capture.
Flow Cytometry⁴: The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1a -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunohistochemistry³. The 364-3B3-14 is a non-neutralizing antibody.

Application References

(PubMed link indicates BioLegend citation)

1. Thorpe R, et al. 1988. Lymphokine Res. 2:119.
2. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
3. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
4. Enriquez, J, et al. 2002. Adv. Perit Dial. 18:177.
5. Lu Y, et al. 2015. PNAS. 112:607. PubMed

RRID

AB_2890851 (BioLegend Cat. No. 500108)
AB_2890851 (BioLegend Cat. No. 500109)

Antigen Details

Structure

Cytokine; 17.5 kD (Mammalian)

Bioactivity

Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia

Cell Sources

Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,

Cell Targets

B cells, T cells, monocytes, dendritic cells

Receptors

Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)

Biology Area

Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bomford, R., et al. Eds. 1989. Interleukin-1, inflammation and disease. Elsevier, New York.
3. Brazel, D., et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello, C. 1996. Blood 87:2095.

Regulation

Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α₂-macroglobulin

Gene ID

3552 View all products for this Gene ID

UniProt

View information about IL-1alpha on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Active Protocols: Sandwich ELISA - Video
• ELISPOT Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis