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Ultra-LEAF™ Purified anti-mouse CD115 (CSF-1R) Antibody

Pricing & Availability
Clone
W20028C (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage colony-stimulating factor 1 receptor, M-CSFR, M-CSF-R, CSF-1 receptor, CSF-1R, CSF-1-R, CSF1R, Proto-oncogene c-Fms, FMS, CD115
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
W20028C_ULEAF_CD115_CSF-1R_Antibody_090821.png
Recombinant mouse M-CSF (Cat. No. 576404) (black circles) induces the proliferation of M-NFS-60 cells. Ultra-LEAF™ purified anti-mouse CD115 (CSF-1R) antibody (W20028C) (purple squares) inhibits the effect of recombinant mouse M-CSF at 0.02 µg/mL on M-NFS-60 cells in a dose-dependent manner whereas the purified rat IgG2b, λ isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 10 - 50 ng/mL.
  • W20028C_ULEAF_CD115_CSF-1R_Antibody_090821.png
    Recombinant mouse M-CSF (Cat. No. 576404) (black circles) induces the proliferation of M-NFS-60 cells. Ultra-LEAF™ purified anti-mouse CD115 (CSF-1R) antibody (W20028C) (purple squares) inhibits the effect of recombinant mouse M-CSF at 0.02 µg/mL on M-NFS-60 cells in a dose-dependent manner whereas the purified rat IgG2b, λ isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 10 - 50 ng/mL.
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946303 100 µg $259
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946304 1 mg $765
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitinylation of intracellular residues. The natural ligands of CD115 include M-CSF and IL-34. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CSF-1R (CD115)-Fc chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant mouse M-CSF at 20 ng/mL on M-NFS-60 cells. ND50 range: 10 - 50 ng /mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2904455 (BioLegend Cat. No. 946303)
AB_2904455 (BioLegend Cat. No. 946304)

Antigen Details

Structure
Disulfide linked homodimer
Distribution

Primitive multipotent hematopoietic cells, monocytes/macrophages, plasmacytoid, conventional dendritic cells, B cells, smooth muscle cells, neuron, osteoclasts and some cancer cells, plasma membrane

Function
CSF-1R is involved in regulation of survival, proliferation and differentiation of hematopoietic progenitor cells. It is also involved in innate immunity and inflammation.
Interaction
Dendritic cells, Macrophages, Monocytes, Osteoclasts
Ligand/Receptor
Macrophage Colony Stimulating Factor (M-CSF) and IL-34
Cell Type
Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Sapi E, et al. 1995. Oncogene. 10: 529.
  2. Dai XM, et al. 2002. Blood. 99: 111.
  3. Lin EY, et al. 2001. J Exp Med. 193: 727.
  4. Gill K, et al. 2001. Fertil Steril. 76: 1005.
  5. Flick MB, et al. 2002. J Cell Biochem. 85: 10.
  6. Chihara T, et al. 2010. Cell Death Differ. 17: 1917.
  7. Chitu V, et al. 2006. Curr Opin Immunol. 18: 39.
  8. Wei S, et al. 2010. J Leukoc Biol. 88: 495.
  9. Yeung YG, et al. 1998. J Biol Chem. 273: 17128.
  10. Chambers SK, et al. 1994. Steroids. 59: 514.
  11. Ide H, et al. 2002. Proc Natl Acad Sci USA. 99: 14404.
  12. Ohno H, et al. 2006. Mol Cancer Ther. 5: 2634.
  13. Rovida E, et al. 2001. J Immunol. 166: 1583.
  14. Wrobel CN, et al. 2004. J Cell Biol. 165: 263.
  15. Cannarile MA, et al. 2017. J Immunother Cancer. 5: 53.
  16. Guo J, et al. 2006. Mol Cancer Ther. 5: 1007.
  17. Hiraga T, et al. 2009. Int J Cancer. 124: 215.
  18. Machkani B, et al. 2010. Bioorg Med Chem. 18: 1789.
  19. Battisti C, et al. 2014. J Neurol. 261: 768.
Gene ID
12978 View all products for this Gene ID
UniProt
View information about CD115 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/08/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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