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Ultra-LEAF™ Purified anti-mouse IL-2 Recombinant Antibody

Pricing & Availability
Clone
QA19A06 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-4, B-cell stimulating factor-1 (BSF-1), Ia inducing factor (IaIF), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A06_ULEAF_IL-2_RECOM_Antibody_113020.png
Recombinant mouse IL-2 (black circles) (Cat. No. 575406) induces the proliferation of HT-2 mouse cells. Anti-mouse IL-2 recombinant antibody (purple squares) (clone QA19A06) inhibits the effect of recombinant mouse IL-2 at 10 ng/mL in HT-2 mouse cells in a dose-dependent manner whereas the isotype control (gray triangles) (Cat. No. 400193) does not have the effect. The ND50 = 0.2 - 1 µg/mL.
  • QA19A06_ULEAF_IL-2_RECOM_Antibody_113020.png
    Recombinant mouse IL-2 (black circles) (Cat. No. 575406) induces the proliferation of HT-2 mouse cells. Anti-mouse IL-2 recombinant antibody (purple squares) (clone QA19A06) inhibits the effect of recombinant mouse IL-2 at 10 ng/mL in HT-2 mouse cells in a dose-dependent manner whereas the isotype control (gray triangles) (Cat. No. 400193) does not have the effect. The ND50 = 0.2 - 1 µg/mL.
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937903 100 µg $270
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937904 1 mg $715
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant mouse IL-2
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by mouse recombinant IL-2 (10 ng/mL) on mouse HT-2 cells. ND50 range: 0.2 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This recombinant antibody clone is derived from clone JES6-1A12 .

RRID
AB_2888842 (BioLegend Cat. No. 937903)
AB_2888842 (BioLegend Cat. No. 937904)

Antigen Details

Structure
Cytokine
Distribution

T cells

Function
Upregulated by NFAT; downregulated by TCF-8, CIF (colostrum inhibitory factor)
Interaction
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Ligand/Receptor
High affinity heterotrimer of IL-2Rα/β/γl, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immuno-Oncology, Immunology, Inhibitory Molecules, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Detanico T, et al. 2011. J. Immunol. 187:82.
  2. Malu DT, et al. 2011. J. Immunol. 186:6271.
  3. Alcaide P, et al. 2012. J. Immunol. 188:1421.
  4. Williams JW, et al. 2013. Am. J. Physiol. Lung Cell Mol. Physiol. 305:693.
Gene ID
16183 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 03/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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