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Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl Antibody

Pricing & Availability
Clone
HTK888 (See other available formats)
Regulatory Status
RUO
Isotype
Armenian Hamster IgG
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
400969 100 µg $110
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400940 1 mg $149
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400959 5 mg $539
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400960 25 mg $1408
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400967 50 mg $2354
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400968 100 mg $3383
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Trinitrophenol + KLH
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC, ICFC - Quality tested
ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400940) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Lesley R, et al. 2006. P. Natl. Acad. Sci. USA 103:10717.
  2. Yu R, et al. 2006. Obesity 14:1353.
  3. Yang JH, et al.2005. Rheumatology(Oxford). 44:1245. PubMed
  4. Mina-Osorio P, et al. 2008. J. Leukocyte Biol. 84:448. PubMed
  5. Shen H, et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed
Product Citations
  1. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  2. Tang Y, et al. 2022. Dev Cell. 57:480. PubMed
  3. Arnholdt C, et al. 2022. Cells. 11:. PubMed
  4. Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed
  5. Gounder AP, et al. 2018. PLoS Pathog. 14:e1007001. PubMed
  6. Gyorke CE, et al. 2020. J Immunol. 205:3037. PubMed
  7. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  8. Deng KM, et al. 2021. Cells. 10:. PubMed
  9. Ross EC, et al. 2021. Cell Mol Life Sci. 78:5197. PubMed
  10. Olafsson EB, et al. 2019. J Cell Sci. 132. PubMed
  11. Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed
  12. Ji DX, et al. 2019. Nat Microbiol. 1.644444444. PubMed
  13. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  14. Zhou HM, et al. 2020. Exp Dermatol. 29:102. PubMed
  15. He C, et al. 2022. JCI Insight. 7:. PubMed
  16. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
RRID
AB_11203529 (BioLegend Cat. No. 400969)
AB_11203529 (BioLegend Cat. No. 400940)
AB_11203529 (BioLegend Cat. No. 400959)
AB_11203529 (BioLegend Cat. No. 400960)
AB_11203529 (BioLegend Cat. No. 400967)
AB_11203529 (BioLegend Cat. No. 400968)

Antigen Details

Gene ID
NA

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Biotin Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
FITC Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
PE Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
PE/Cyanine5 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Purified Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC,IP,ICC,IHC,WB,FA,ChIP
PE/Cyanine7 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Alexa Fluor® 488 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Alexa Fluor® 647 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Pacific Blue™ Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Alexa Fluor® 700 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
APC/Cyanine7 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
PerCP Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
PerCP/Cyanine5.5 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Brilliant Violet 421™ Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Brilliant Violet 570™ Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC,ICC,IHC,IP,WB,FA
Brilliant Violet 510™ Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Brilliant Violet 605™ Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Brilliant Violet 650™ Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Brilliant Violet 785™ Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
PE/Dazzle™ 594 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Alexa Fluor® 594 Armenian Hamster IgG Isotype Ctrl HTK888 ICC
APC/Fire™ 750 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Brilliant Violet 711™ Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
GoInVivo™ Purified Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC,ICC,IHC,IP,WB,FA
TotalSeq™-A0241 Armenian Hamster IgG Isotype Ctrl HTK888 PG
KIRAVIA Blue 520™ Armenian Hamster IgG Isotype Ctrl HTK888 FC
TotalSeq™-C0241 Armenian Hamster IgG Isotype Ctrl HTK888 PG
TotalSeq™-B0241 Armenian Hamster IgG Isotype Ctrl HTK888 PG
Spark YG™ 570 Armenian Hamster IgG Isotype Ctrl HTK888 FC
Spark Red™ 718 Armenian Hamster IgG Isotype Ctrl HTK888 FC,ICFC
Spark Blue™ 515 Armenian Hamster IgG Isotype Ctrl HTK888 FC
PerCP/Fire™ 780 Armenian Hamster IgG Isotype Ctrl HTK888 FC
Spark Blue™ 574 Armenian Hamster IgG Isotype Ctrl HTK888 FC
Spark PLUS UV395™ Armenian Hamster IgG Isotype Ctrl HTK888 FC
Go To Top Version: 3    Revision Date: 08/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Armenian Hamster IgG Isotype Ctrl

  • Biotin Armenian Hamster IgG Isotype Ctrl

  • FITC Armenian Hamster IgG Isotype Ctrl

  • PE Armenian Hamster IgG Isotype Ctrl

  • PE/Cyanine5 Armenian Hamster IgG Isotype Ctrl

  • Purified Armenian Hamster IgG Isotype Ctrl

    1_HTK888_Purified_IgG_Antibody_021920
    Whole cell extracts (190 µg total protein) prepared from Jur...
    2_HTK888_GoChIP_IgG_Antibody_1_082619.png
    Chromatin Immunoprecipitations (ChIP) were done with fixed a...

  • PE/Cyanine7 Armenian Hamster IgG Isotype Ctrl

  • Alexa Fluor® 488 Armenian Hamster IgG Isotype Ctrl

  • Alexa Fluor® 647 Armenian Hamster IgG Isotype Ctrl

  • Pacific Blue™ Armenian Hamster IgG Isotype Ctrl

  • Alexa Fluor® 700 Armenian Hamster IgG Isotype Ctrl

  • APC/Cyanine7 Armenian Hamster IgG Isotype Ctrl

  • PerCP Armenian Hamster IgG Isotype Ctrl

  • PerCP/Cyanine5.5 Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 421™ Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 570™ Armenian Hamster IgG Isotype Ctrl

  • Ultra-LEAF™ Purified Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 510™ Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 605™ Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 650™ Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 785™ Armenian Hamster IgG Isotype Ctrl

  • PE/Dazzle™ 594 Armenian Hamster IgG Isotype Ctrl

  • Alexa Fluor® 594 Armenian Hamster IgG Isotype Ctrl

  • APC/Fire™ 750 Armenian Hamster IgG Isotype Ctrl

  • Brilliant Violet 711™ Armenian Hamster IgG Isotype Ctrl

  • GoInVivo™ Purified Armenian Hamster IgG Isotype Ctrl

  • TotalSeq™-A0241 Armenian Hamster IgG Isotype Ctrl

  • KIRAVIA Blue 520™ Armenian Hamster IgG Isotype Ctrl

  • TotalSeq™-C0241 Armenian Hamster IgG Isotype Ctrl

  • TotalSeq™-B0241 Armenian Hamster IgG Isotype Ctrl

  • Spark YG™ 570 Armenian Hamster IgG Isotype Ctrl

  • Spark Red™ 718 Armenian Hamster IgG Isotype Ctrl

  • Spark Blue™ 515 Armenian Hamster IgG Isotype Ctrl

  • PerCP/Fire™ 780 Armenian Hamster IgG Isotype Ctrl

  • Spark Blue™ 574 Armenian Hamster IgG Isotype Ctrl

  • Spark PLUS UV395™ Armenian Hamster IgG Isotype Ctrl

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