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Ultra-LEAF™ Purified Rat IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2071 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
400431 100 µg $94
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400432 1 mg $149
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400457 5 mg $539
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400458 25 mg $1408
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400461 50 mg $2354
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400462 100 mg $3383
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

The isotype of RTK2071 immunoglobulin is rat IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
ICFC, ICC, IHC, WB, IP, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this rat IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Application Notes

The RTK2071 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400414) as negative control1. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400432) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Riemann M, et al. 2005. J. Immunol. 175:3560. PubMed
  2. Wondimu Z, et al. 2010. Am. J. Pathol. 177:2334. PubMed
Product Citations
  1. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  2. Yamamoto A, et al. 2021. FASEB J. 35:e21158. PubMed
  3. Cui P, et al. 2023. J Neuroinflammation. 20:63. PubMed
  4. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  5. Cheon SY, et al. 2022. J Invest Dermatol. 2668:142. PubMed
  6. Parashar K, et al. 2020. Immun Inflamm Dis. 0.696527778. PubMed
  7. Zhao K, et al. 2022. Adv Sci (Weinh). 9:e2103838. PubMed
  8. Nie X et al. 2018. Neuron. 99(3):464-479 . PubMed
  9. Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed
  10. Mock JR, et al. 2020. Am J Respir Cell Mol Biol. 63:464. PubMed
  11. Shute A, et al. 2021. Microbiome. 9:186. PubMed
  12. Jung H, et al. 2019. Autophagy. 15:1990. PubMed
  13. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  14. Yao M, et al. 2022. Dis Markers. 2022:4253566. PubMed
  15. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  16. Murdock B, et al. 2014. J Immunol. 193:4107. PubMed
RRID
AB_11150233 (BioLegend Cat. No. 400431)
AB_11150772 (BioLegend Cat. No. 400432)
AB_11150233 (BioLegend Cat. No. 400457)
AB_11150233 (BioLegend Cat. No. 400458)
AB_11150233 (BioLegend Cat. No. 400461)
AB_11150233 (BioLegend Cat. No. 400462)

Antigen Details

Gene ID
NA

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Biotin Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
FITC Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
PE Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
PE/Cyanine5 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Purified Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC,ICC,IHC,IP,WB
PE/Cyanine7 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Alexa Fluor® 647 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Alexa Fluor® 488 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Pacific Blue™ Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Alexa Fluor® 700 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
APC/Cyanine7 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
PerCP Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
PerCP/Cyanine5.5 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Brilliant Violet 421™ Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Ultra-LEAF™ Purified Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC,ICC,IHC,WB,IP,FA
Brilliant Violet 605™ Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Brilliant Violet 510™ Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Brilliant Violet 650™ Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Brilliant Violet 711™ Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Brilliant Violet 785™ Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
PE/Dazzle™ 594 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Alexa Fluor® 594 Rat IgG1, κ Isotype Ctrl RTK2071 IHC-F
GoInVivo™ Purified Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC,ICC,IHC,WB,IP,FA
APC/Fire™ 750 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
TotalSeq™-A0236 Rat IgG1, κ Isotype Ctrl RTK2071 PG
KIRAVIA Blue 520™ Rat IgG1, κ Isotype Ctrl RTK2071 FC
TotalSeq™-B0236 Rat IgG1, κ Isotype Ctrl RTK2071 PG
TotalSeq™-C0236 Rat IgG1, κ Isotype Ctrl RTK2071 PG
Spark YG™ 581 Rat IgG1, κ Isotype Ctrl RTK2071 FC
Spark YG™ 593 Rat IgG1, κ Isotype Ctrl RTK2071 FC
Spark NIR™ 685 Rat IgG1, κ Isotype Ctrl RTK2071 FC
PE/Fire™ 640 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
TotalSeq™-D0236 Rat IgG1, κ Isotype Ctrl RTK2071 PG
PE/Fire™ 700 Rat IgG1, κ Isotype Ctrl RTK2071 FC
APC/Fire™ 810 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Brilliant Violet 750™ Rat IgG1, κ Isotype Ctrl RTK2071 FC
Spark YG™ 570 Rat IgG1, κ Isotype Ctrl RTK2071 FC
PerCP/Fire™ 806 Rat IgG1, κ Isotype Ctrl RTK2071 FC
PerCP/Fire™ 780 Rat IgG1, κ Isotype Ctrl RTK2071 FC
Spark Red™ 718 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
TotalSeq™-Bn0236 Rat IgG1, κ Isotype Ctrl RTK2071 SB
PE/Fire™ 744 Rat IgG1, κ Isotype Ctrl RTK2071 FC,ICFC
Go To Top Version: 2    Revision Date: 01/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG1, κ Isotype Ctrl

  • Biotin Rat IgG1, κ Isotype Ctrl

  • FITC Rat IgG1, κ Isotype Ctrl

  • PE Rat IgG1, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG1, κ Isotype Ctrl

  • Purified Rat IgG1, κ Isotype Ctrl

  • PE/Cyanine7 Rat IgG1, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG1, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG1, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG1, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG1, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG1, κ Isotype Ctrl

  • PerCP Rat IgG1, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG1, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG1, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Rat IgG1, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG1, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG1, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG1, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG1, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG1, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG1, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG1, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG1, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG1, κ Isotype Ctrl

  • TotalSeq™-A0236 Rat IgG1, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG1, κ Isotype Ctrl

  • TotalSeq™-B0236 Rat IgG1, κ Isotype Ctrl

  • TotalSeq™-C0236 Rat IgG1, κ Isotype Ctrl

  • Spark YG™ 581 Rat IgG1, κ Isotype Ctrl

  • Spark YG™ 593 Rat IgG1, κ Isotype Ctrl

  • Spark NIR™ 685 Rat IgG1, κ Isotype Ctrl

  • PE/Fire™ 640 Rat IgG1, κ Isotype Ctrl

  • TotalSeq™-D0236 Rat IgG1, κ Isotype Ctrl

  • PE/Fire™ 700 Rat IgG1, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG1, κ Isotype Ctrl

  • Brilliant Violet 750™ Rat IgG1, κ Isotype Ctrl

  • Spark YG™ 570 Rat IgG1, κ Isotype Ctrl

  • PerCP/Fire™ 806 Rat IgG1, κ Isotype Ctrl

  • PerCP/Fire™ 780 Rat IgG1, κ Isotype Ctrl

  • Spark Red™ 718 Rat IgG1, κ Isotype Ctrl

  • TotalSeq™-Bn0236 Rat IgG1, κ Isotype Ctrl

  • PE/Fire™ 744 Rat IgG1, κ Isotype Ctrl

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