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- Clone
- A17002E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- V-Set Immunoregulatory Receptor (VSIR), V-Domain Ig Suppressor of T Cell Activation (VISTA), Stress-Induced Secreted Protein-1 (SISP1), PDCD1 Homolog, Platelet Receptor GI24 (GI24), Death Domain1alpha, DD1 alpha, B7-H5
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood monocytes were stained with Ultra-LEAF™ purified anti-human VISTA (PD-1H) (clone A17002E) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 380003 | 100 µg | $270 | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
VISTA (V-domain Ig suppressor of T cell activation), also known as PD-1H, is a type I transmembrane protein with a single extracellular IgV domain. VISTA is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It was reported that in certain tumor environment, there is low-pH dependent interaction between VISTA and PSGL1. However, the physiological binding partner for VISTA and the mechanism of its action are not clearly understood. VISTA is considered as a checkpoint regulator and a promising target for cancer immunotherapy.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human VISTA protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone A17002E can completely block the binding of clone A17002B on target cells.
Clone A17002E can reduce T cell proliferation in an in vitro T cell activation assay. - RRID
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AB_2928033 (BioLegend Cat. No. 380003)
Antigen Details
- Structure
- Ig V-domain like
- Distribution
-
Hematopoietic cells, monocytes, macrophages, dendritic cell, granulocytes, subsets of T cells
- Function
- Suppressing T cell activation
- Cell Type
- Dendritic cells, Granulocytes, Lymphocytes, Macrophages, Monocytes, T cells
- Biology Area
- Immunology, Inhibitory Molecules
- Antigen References
-
- Wang Li, et al. 2011. J Exp Med. 208:577-92.
- Nowak EC, et al. 2017. Immunol Rev. 276:66-79.
- Flies DB, et al. 2014. J Clin Invest. 124:1966-75.
- Gene ID
- 64115 View all products for this Gene ID
- UniProt
- View information about VISTA on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All VISTA Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human VISTA (PD-1H) | A17002E | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human VISTA (PD-1H)
Human peripheral blood monocytes were stained with Ultra-LEA...
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