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Western-Ready™ Transfer Buffer (10X)

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426313 1 L $59
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Technical data sheet

Product Details

Formulation
Proprietary
Preparation
This buffer is bottled as 10X. To prepare a working solution (1X) dilute Western-Ready™ Transfer Buffer (10X) to 1X concentration (1:10 by volume). 1 part of Western-Ready™ Transfer Buffer (10X), 2 parts of 100% methanol, and 7 parts of DI water.
Storage & Handling
Upon receipt, store solutions at room temperature. Products are shipped at ambient (room) temperature.
Application

WB - Quality tested
Recommended Usage

Dilute Western-Ready™ Transfer Buffer (10X) to 1X concentration (1:10 by volume). 1 part of Western-Ready™ Transfer Buffer (10X), 2 parts of 100% methanol, and 7 parts of DI water.

Application Notes

This buffer is formulated for Western blot protein transfer. By direct PDVF membrane staining using Licor Revert 700 protein dye, we are able to detect as low as 25 ng/band on high and medium molecular weight proteins, and as low as 12.5 ng/band in low molecular weight proteins. The buffer is validated for protein transfer to both nitrocellulose and PVDF membranes. This buffer is only recommended for wet  protein transfers.

Western-Ready™ Transfer Buffer does not include any methanol.

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/04/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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