Alexa Fluor® 488 anti-BrdU Antibody

Pricing & Availability
Clone
3D4 (See other available formats)
Regulatory Status
RUO
Other Names
5-bromodeoxyuridine, bromodeoxyuridine
Isotype
Mouse IgG1, κ
3D4_AF488_BrdU_Antibody_ICFC_1_112514
Human T lymphoblastic leukemia cell line, Hut-78, was pulsed with BrdU for one hour, fixed and permeabilized with cold 70% ethanol, and then stained with anti-BrdU (clone 3D4) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom).
  • 3D4_AF488_BrdU_Antibody_ICFC_1_112514
    Human T lymphoblastic leukemia cell line, Hut-78, was pulsed with BrdU for one hour, fixed and permeabilized with cold 70% ethanol, and then stained with anti-BrdU (clone 3D4) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom).
  • 3D4_AF488_BrdU_Antibody_ICFC_2_112514
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability
364105 25 tests $112.00
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364106 100 tests $268.00
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Description

BrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.

Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Iodouridine-conjugated ovalbumin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.

Application References

(PubMed link indicates BioLegend citation)
  1. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
  2. Hirota K, et al. 2007. J. Exp. Med. 204:41.
  3. Godebu E, et al. 2008. J. Immunol. 181:1798.
  4. Waskow C, et al. 2008. Nat. Immunol. 9:676.
Product Citations
  1. Kaymak A, et al. 2018. Oncotarget. 9:28666. PubMed
  2. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  3. Berges C, et al. 2015. J Leukoc Biol. 98: 1091 - 1105. PubMed
  4. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  5. Davenne T, et al. 2020. Cell Rep. 31:107640. PubMed
  6. Li H et al. 2019. Developmental cell. 49(1):118-129 . PubMed
  7. Li P, et al. 2022. EMBO Mol Med. 14:e14511. PubMed
  8. Radke J, et al. 2022. Nat Commun. 13:7304. PubMed
RRID
AB_2564499 (BioLegend Cat. No. 364105)
AB_2564499 (BioLegend Cat. No. 364106)

Antigen Details

Structure
Uridine derivative that can be incorporated into DNA and substitute thymidine residues.
Distribution

Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.

Function
The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Barker JM, et al. 2013. PLoS One 8:e63692.
2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205.
3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855.
4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954.
5. van der Wath RC, et al. 2009. PLoS One 4:e6972.
6. Dolbeare F, et al. 1985. Cytometry 6:521.
7. Gratzner HG. 1982. Science 218:474.

Gene ID
NA
UniProt
View information about BrdU on UniProt.org
Go To Top Version: 1    Revision Date: 12/01/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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