Brilliant Violet 510™ anti-BrdU Antibody

Product Details

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Iodouridine-conjugated ovalbumin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.

Application References

(PubMed link indicates BioLegend citation)

1. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
2. Hirota K, et al. 2007. J. Exp. Med. 204:41.
3. Godebu E, et al. 2008. J. Immunol. 181:1798.
4. Waskow C, et al. 2008. Nat. Immunol. 9:676.

RRID

AB_3105984 (BioLegend Cat. No. 364119)
AB_3105984 (BioLegend Cat. No. 364120)

Antigen Details

Structure

Uridine derivative that can be incorporated into DNA and substitute thymidine residues.

Distribution

Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.

Function

The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.

Biology Area

Cell Biology, Cell Cycle/DNA Replication, Immunology

Molecular Family

Nuclear Markers

Antigen References

1. Barker JM, et al. 2013. PLoS One 8:e63692.
2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205.
3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855.
4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954.
5. van der Wath RC, et al. 2009. PLoS One 4:e6972.
6. Dolbeare F, et al. 1985. Cytometry 6:521.
7. Gratzner HG. 1982. Science 218:474.

Gene ID

NA

UniProt

View information about BrdU on UniProt.org

Related Protocols

• Anti-BrdU Staining Using 70% Ethanol and 2N HCL
• Anti-BrdU Staining Using DNAse with Surface and Fluorescent Proteins

Other Formats