Alexa Fluor® 488 anti-human IgD Antibody

Pricing & Availability
Clone
IA6-2 (See other available formats)
Regulatory Status
RUO
Other Names
Ig delta chain C region
Isotype
Mouse IgG2a, κ
1_IA6-2_AF488_1_022712
Human peripheral blood lymphocytes were stained with CD19 Brilliant Violet 421™ and IgD (clone IA6-2) Alexa Fluor® 488 (top) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
  • 1_IA6-2_AF488_1_022712
    Human peripheral blood lymphocytes were stained with CD19 Brilliant Violet 421™ and IgD (clone IA6-2) Alexa Fluor® 488 (top) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
  • 2_IA6-2_AF488_2_022712
  • 3_Human_LN_IgD_PD-1
    Confocal image of human lymph node sample acquired using the IBEX method of highly multiplexed antibody-based imaging: IgD (blue) in Cycle 2, PD-1 (green) in Cycle 5. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability
348215 25 tests $118.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
348216 100 tests $265.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IgD
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections4 and spatial biology (IBEX)5,6.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Chen K, et al. 2009. Nat. Immunol. 10:889.
  2. Lee CH, et al. 2005. J. Exp. Med. 203:63.
  3. Sutter JA, et al. 2008. Clin. Immunol. 126:282.
  4. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  2. Chen HY, et al. 2022. Elife. 11:. PubMed
  3. Harrington WE, et al. 2021. Cell Reports Medicine. 2(4):100253. PubMed
  4. Powell WE, et al. 2018. Diabetologia. 61:1794. PubMed
  5. Golovkin A, et al. 2021. Viruses. 13:. PubMed
  6. Shin JJ, et al. 2022. J Clin Immunol. :. PubMed
  7. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
  8. Kudryavtsev IV, et al. 2021. Curr Issues Mol Biol. 44:194. PubMed
  9. Yang L, et al. 2020. Genes Dis. 7:128. PubMed
  10. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  11. Fisher BS, et al. 2020. PLoS One. 15:e0233577. PubMed
RRID
AB_11150595 (BioLegend Cat. No. 348215)
AB_11150595 (BioLegend Cat. No. 348216)

Antigen Details

Structure
Exists in a transmembranal and a soluble form
Distribution

Naïve B cells

Function
Antigen binding, B cell activation
Interaction
The CD79a/CD79b heterodimer
Cell Type
B cells
Biology Area
Immunology
Antigen References

1. Geisberger R, et al. 2006. Immunology 118:429.
2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789.
3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32.

Gene ID
3495 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 5    Revision Date: 04/21/2022

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account