Alexa Fluor® 700 anti-human IgD Antibody

Pricing & Availability
Clone
IA6-2 (See other available formats)
Regulatory Status
RUO
Other Names
Ig delta chain C region
Isotype
Mouse IgG2a, κ
IA6-2_AF700_IgD_Antibody_FC_1_032114.jpg
Human peripheral blood lymphocytes were stained with CD19 PE and IgD (clone IA6-2) Alexa Fluor® 700 (top) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (bottom).
  • IA6-2_AF700_IgD_Antibody_FC_1_032114.jpg
    Human peripheral blood lymphocytes were stained with CD19 PE and IgD (clone IA6-2) Alexa Fluor® 700 (top) or mouse IgG2a, κ Alexa Fluor® 700 isotype control (bottom).
  • IA6-2_AF700_IgD_Antibody_FC_2_032114.jpg
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
348229 25 tests $159.00
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348230 100 tests $358.00
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Description

IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IgD
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections4 and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Chen K, et al. 2009. Nat. Immunol. 10:889.
  2. Lee CH, et al. 2005. J. Exp. Med. 203:63.
  3. Sutter JA, et al. 2008. Clin. Immunol. 126:282.
  4. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Meehan S, et al. 2019. Commun Biol. 2:229. PubMed
  2. van Heerden D, et al. 2021. Int J Mol Sci. 22:. PubMed
  3. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  4. Khoo NKH, et al. 2022. Med (N Y). 3:104. PubMed
  5. Chen Y, et al. 2020. Cell. 1496:183. PubMed
  6. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  7. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
RRID
AB_2563334 (BioLegend Cat. No. 348229)
AB_2563334 (BioLegend Cat. No. 348230)

Antigen Details

Structure
Exists in a transmembranal and a soluble form
Distribution

Naïve B cells

Function
Antigen binding, B cell activation
Interaction
The CD79a/CD79b heterodimer
Cell Type
B cells
Biology Area
Immunology
Antigen References

1. Geisberger R, et al. 2006. Immunology 118:429.
2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789.
3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32.

Gene ID
3495 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 4    Revision Date: 09/02/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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