Alexa Fluor® 488 anti-mouse CD3ε Antibody

Pricing & Availability
Clone
500A2 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Syrian Hamster IgG
1_500A2_A488_CD3-epsilon_Antibody_1_050619
C57BL/6 splenocytes were stained with CD19 APC/Cyanine7 and CD3ε (clone 500A2) Alexa Fluor® 488 (left) or Syrian Hamster IgG isotype control (right) Alexa Fluor® 488.
  • 1_500A2_A488_CD3-epsilon_Antibody_1_050619
    C57BL/6 splenocytes were stained with CD19 APC/Cyanine7 and CD3ε (clone 500A2) Alexa Fluor® 488 (left) or Syrian Hamster IgG isotype control (right) Alexa Fluor® 488.
  • 2_500A2_A488_CD3-epsilon_Antibody_101818
    Balb/C mouse frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 488 anti-mouse CD3 Antibody (clone 500A2, green), Alexa Fluor® 594 anti-mouse CD11b Antibody (clone M1/70, red) and Alexa Fluor® 647 anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2, blue) overnight at 4°C. Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 3_500A2_A488_CD3epsilon_Antibody_3_091721.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse CD3ε Antibody (clone 500A2) Alexa Fluor® 488 at 5 µg/mL (green), and anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2) Alexa Fluor® 594 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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Cat # Size Price Quantity Check Availability
152321 25 µg $124.00
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152322 100 µg $223.00
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
Mouse T cell receptor.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.

Application References

(PubMed link indicates BioLegend citation)
  1. Miescher GC, et al. 1989. Immunol. Lett. 23:113.
RRID
AB_2783124 (BioLegend Cat. No. 152321)
AB_2783124 (BioLegend Cat. No. 152322)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Distribution

Thymocytes (differentiation dependent), mature T cells and NK-T cells.

Function
TCR signal transduction, T cell activation and antigen recognition.
Interaction
T-cell receptor (TCR).
Ligand/Receptor
Peptide antigen/MHC-complex.
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell. 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 3    Revision Date: 09/17/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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