Alexa Fluor® 700 anti-mouse CD3ε Antibody

Pricing & Availability
Clone
500A2 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Syrian Hamster IgG
500A2_AF700_CD3epsilon_Antibody_103116
C57BL/6 splenocytes were stained with CD19 FITC and CD3ε (clone 500A2) Alexa Fluor® 700.
  • 500A2_AF700_CD3epsilon_Antibody_103116
    C57BL/6 splenocytes were stained with CD19 FITC and CD3ε (clone 500A2) Alexa Fluor® 700.
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
152315 25 µg $118.00
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152316 100 µg $211.00
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
Mouse T cell receptor.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.

Application References

(PubMed link indicates BioLegend citation)
  1. Miescher GC, et al. 1989. Immunol. Lett. 23:113.
Product Citations
  1. Jacquelot N, et al. 2022. STAR Protoc. 3:101534. PubMed
  2. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
  3. Roy R, et al. 2022. Elife. 11:. PubMed
  4. Poillet-Perez L, et al. 2020. Nat Cancer. 1:923. PubMed
  5. Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
  6. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  7. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
RRID
AB_2632712 (BioLegend Cat. No. 152315)
AB_2632712 (BioLegend Cat. No. 152316)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Distribution

Thymocytes (differentiation dependent), mature T cells and NK-T cells.

Function
TCR signal transduction, T cell activation and antigen recognition.
Interaction
T-cell receptor (TCR).
Ligand/Receptor
Peptide antigen/MHC-complex.
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell. 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 1    Revision Date: 10/31/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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