Alexa Fluor® 488 anti-rat CD25 Antibody

Pricing & Availability
Clone
OX-39 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2 receptor chain α, IL-2Rα, low affinity IL-2 receptor chain, Tac, p55, Ly-43
Isotype
Mouse IgG1, κ
OX-39_Alx488_090707
Con A-activated (3-days) LOU rat splenocytes stained with OX-39 Alexa Fluor® 488
  • OX-39_Alx488_090707
    Con A-activated (3-days) LOU rat splenocytes stained with OX-39 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability
202108 100 µg $229.00
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Description

CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.

Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat T cell blasts from mixed lymphocyte reactions
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1, immunoprecipitation1, weakly blocks IL-2 binding1, 2, 3, and blocks IL-2 induced epithelial cell migration3.

Application References

(PubMed link indicates BioLegend citation)
  1. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281. (IHC, IP, Block)
  2. Tellides G, et al. 1987. Transplant Proc. 19:4231. (Block)
  3. Digness AU, et al. 1996. Exp.Cell Res. 225:422. (Block)
Product Citations
  1. Chen KH, et al. 2019. J Neuropathol Exp Neurol. 78:65. PubMed
RRID
AB_2125458 (BioLegend Cat. No. 202108)

Antigen Details

Structure
Type I membrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, thymic and splenic dendritic cells, intestinal epithelial cells

Function
Low affinity receptor for IL-2; associates with IL-2 receptor β (CD122) and γ (CD132) chains to form high affinity receptor
Ligand/Receptor
IL-2
Cell Type
B cells, Dendritic cells, Epithelial cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Digness AU, et al. 1996. Exp.Cell Res. 225:422.
2. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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