- Clone
- OX-39 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2 receptor chain α, IL-2Rα, low affinity IL-2 receptor chain, Tac, p55, Ly-43
- Isotype
- Mouse IgG1, κ
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Con A stimulated (3-days) rat splenocytes were stained with anti-rat CD25 (clone OX-39) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 202115 | 50 µg | $250.00 | |||
CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.
Product Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat T cell blasts from mixed lymphocyte reactions
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1, immunoprecipitation1, weakly blocks IL-2 binding1, 2, 3, and blocks IL-2 induced epithelial cell migration3.
-
Application References
(PubMed link indicates BioLegend citation) -
- Peterson DJ, et al. 1987. Mol. Immunol. 24:1281. (IHC, IP, Block)
- Tellides G, et al. 1987. Transplant Proc. 19:4231. (Block)
- Digness AU, et al. 1996. Exp.Cell Res. 225:422. (Block)
- RRID
-
AB_3662392 (BioLegend Cat. No. 202115)
Antigen Details
- Structure
- Type I membrane glycoprotein, 55 kD
- Distribution
-
Activated T cells and B cells, thymic and splenic dendritic cells, intestinal epithelial cells
- Function
- Low affinity receptor for IL-2; associates with IL-2 receptor β (CD122) and γ (CD132) chains to form high affinity receptor
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Dendritic cells, Epithelial cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Digness AU, et al. 1996. Exp.Cell Res. 225:422.
2. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281. - Gene ID
- 16184 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Other Formats
View All CD25 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| FITC anti-rat CD25 | OX-39 | FC |
| PE anti-rat CD25 | OX-39 | FC |
| Alexa Fluor® 488 anti-rat CD25 | OX-39 | FC |
| Biotin anti-rat CD25 | OX-39 | FC |
| APC anti-rat CD25 | OX-39 | FC |
| Brilliant Violet 421™ anti-rat CD25 | OX-39 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-rat CD25
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PE anti-rat CD25
ConA-stimulated (3day) LOU splenocytes stained with OX-39 PE -
Alexa Fluor® 488 anti-rat CD25
Con A-activated (3-days) LOU rat splenocytes stained with OX... -
Biotin anti-rat CD25
Con A-stimulated LOU rat splnocytes (3days) stained with bio... -
APC anti-rat CD25
Con A activated (3-days) rat splenocytes were stained with C... -
Brilliant Violet 421™ anti-rat CD25
Con A stimulated (3-days) rat splenocytes were stained with ...